Key Specifications Table
| Detection Methods |
|---|
| Fluorescent |
| Description | |
|---|---|
| Catalogue Number | S7113 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | DAPI/Antifade Solution, Ready to Use |
| Overview | DAPI: 4, 6-Diamino-2-phenylindole dihydrochloride. Antifade Solution: p-Phenylenediamine and glycerol in PBS solution. 0.4μg/mL DAPI suspended in Antifade. The product is ready to add to fixed slides or cells cultures. |
| Product Information | |
|---|---|
| Detection method | Fluorescent |
| Presentation | Liquid PBS pH 8.0 containing ~50% glycerol and 0.4μg/mL of DAPI and 2-7mM phenylenediamine. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application Notes | A nuclear fluorescent stain used for counterstaining paraffin-embedded, formalin-fixed tissue, metaphase chromosomes, or interphase nuclei that have been hybridized with probes detected with Fluorescein, or equivalent stains. Antifade prevents the permanent loss of fluorescence or fluorescent signal quenching due to photobleaching and prolonged exposure to high intensity light sources. DAPI binds the minor groove of DNA at AT clusters and binds preferentially to ds DNA (Biochemistry 26:4545, 1987). Materials Provided Each vial contains 1 ml of DAPI/Antifade Solution Warnings and Precautions DAPI is a potential carcinogen. Antifade is a potential toxin and irritant. Use extreme caution. Avoid contact with eyes, skin and mucous membranes. Use extreme caution. Wear gloves and a lab coat when handling. Follow your institutions guidelines for hazardous waste disposal. Keep tightly capped and limit exposure to light. Related Products Antifade Solution S7114 Propidium Iodide Solution S7109 Propidium Iodide/Antifade Solution S7112 |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Store at -20ºC in undiluted aliquots for up to 1 year from date of receipt. Protect from light |
| Packaging Information | |
|---|---|
| Material Size | 1 mL |