| Description | |
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| Catalogue Number | S7842 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | CpG WIZ® H19-IGF2 Amplification Kit |
| Overview | Methylation of cytosines located 5 to guanosine is known to have a profound effect on the expression of several eukaryotic genes (Bird, 1992 ). In normal cells, methylation occurs predominantly in CG-poor regions, while CG-rich areas, called CpG islands, remain unmethylated. Aberrant methylation of normally unmethylated CpG islands has been documented as a relatively frequent event in immortalized and transformed cells (Antequera, 1990) and has been associated with transcriptional inactivation of defined tumor suppressor genes in human cancers (Merlo, 1995). Aberrant methylation of H19-IGF2 locus is implicated in several inherited genetic disorders. Methylation-specific PCR (MSP) is a new technology for sensitive detection of abnormal gene methylation utilizing small amounts of DNA (Herman, 1996 ). This process employs an initial bisulfite reaction to modify the DNA, followed by PCR amplification with specific primers designed to distinguish methylated from unmethylated DNA. |
| Materials Required but Not Delivered | a. Microcentrifuge tubes for PCR amplification b. Aerosol-resistant pipette tips c. Thermocycler d. Gel electrophoresis apparatus (vertical or horizontal) e. Power Supply f. 302 nm UV transilluminator, camera and film Reagents a. 2.5 mm dNTP mix (2.5 mm of each nucleotide) b. "Hot start" Taq polymerase c. "Hot start" PCR reagents (see Sec. II. Protocols). d. Reagents for gel electrophoresis (1X TBE and 2% agarose, 10% acrylamide, or suitable high resolution agarose) e. DNA markers (size range 100-300 bp) f. Ethidium bromide (10 mg/mL) g. Gel-loading solution / Loading Dye H. . Bisulfite Modified DNA (CpGenome™ DNA Modification Kit, Cat. No. S7820; CpGENOME™ Fast DNA Modification Kit, Cat. No. S7824) |
| Product Information | |
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| Components |
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| Quality Level | MQ100 |
| Applications | |
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| Application | The components of the CpG WIZ H19-IGF2 Amplification Kit include those required for PCR amplification after bisulfite modification of DNA samples. |
| Key Applications |
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| Application Notes | Principles of the Technique Use of either the CpGenome™ DNA Modification Kit (Cat. No. S7820) or the CpGENOME™ Fast DNA Modification Kit (Cat. No. S7824) facilitates the initial bisulfite reactions, while the CpG WIZ® H19-IGF2 Amplification Kit contains the reagents required for the gene-specific PCR amplification reactions. |
| Biological Information | |
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| Species Reactivity |
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| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is an imprinted gene and is expressed only from the paternally inherited allele. It is a candidate gene for eating disorders. There is a read-through, INS-IGF2, which aligns to this gene at the 3 region and to the upstream INS gene at the 5 region. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P01344 # The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development. SIZE: 180 amino acids; 20140 Da SUBCELLULAR LOCATION: Secreted. SIMILARITY: SwissProt: P01344 ## Belongs to the insulin family. |
| Product Usage Statements | |
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| Packaging Information | |
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| Material Size | 25 assays |