Key Specifications Table
| Key Applications |
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| Cult |
| Description | |
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| Catalogue Number | SCM004 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | ENStem-A™ Neural Expansion Medium |
| Overview | ENStem-A Neural Expansion Medium is a defined serum-free formulation that has been optimized for the culture and expansion of ENStem-A Human Neural Progenitor Cells. When used in conjunction with L-Glutamine (not provided) and FGF-2 (provided), the expansion medium will allow for the maintenance and proliferation of ENStem-ATM Human Neural Progenitor Cells. |
| Materials Required but Not Delivered | • L-Glutamine (Cat. No. TMS-002-C) • ENStem-A™ Human Neural Progenitor Expansion Kit (Cat. No. SCR055) • ENStem-A™ Neural Freezing Medium (Cat. No. SCM011) • ENStem-A™ Neuronal Differentiation Medium (Cat. No. SCM017) • Poly-L-ornithine hydrobromide (Sigma Part No. P3655) • Mouse Laminin (Sigma Part No. L-2020) • Penicillin/Streptomycin Solution (Cat. No. TMS-AB2-C) • Accutase™ (Cat. No. SCR005) • Phosphate-Buffered Saline (1X PBS) (Cat. No. BSS-1005-B) • Tissue culture-ware • Hemacytometer • Microscope |
| Product Information | |
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| Components |
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| Formulation | |
| Quality Level | MQ100 |
| Applications | |
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| Application | The ENStem-A Neural Expansion Medium is a defined serum-free formulation that has been optimized for the culture & expansion of ENStem-A Human Neural Progenitor Cells. |
| Key Applications |
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| Application Notes | Cell culture protocols for use with ENStem-A™ Human Neural Progenitor Cells are available in the datasheet that accompanies the ENStem-A™ Human Neural Progenitor Expansion Kit (Cat. No. SCR055). |
| Biological Information | |
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| Media Form | Liquid |
| Stem Cell Type |
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| Product Usage Statements | |
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| Quality Assurance | Each lot of medium is tested for the optimal cell growth and proliferation of ENStem-ATM Human Neural Progenitor Cells. Sterility Testing: Negative |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | ENStem-ATM Neural Expansion Medium should be stored at -20°C until ready to use. Upon thawing, fresh L-Glutamine (not provided) should be added for a final concentration of 2 mM to the expansion medium. Thawed should be stored at 2-8°C and given a 1-month expiration dating. Dispense into aliquots to avoid repeated heating prior to each use. FGF-2 (10 μg, lyophilized) should be reconstituted with 100 μL 5 mM Tris-HCL, pH 7.6 for a final concentration of 100 μg/mL. Dispense into aliquots to avoid repeated freeze-thaw. Store at -20°C. |
| Packaging Information | |
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| Material Size | 500 mL |