Key Specifications Table
| Key Applications |
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| Cult |
| Description | |
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| Catalogue Number | SCM005 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | ReNcell® NSC Maintenance Media |
| Overview | ReNcell® NSC Maintenance Medium is a defined serum-free, growth factor-free medium that has been optimized for the growth and in vitro differentiation of ReNcell® immortalized human neural progenitor cells. When used in conjunction with FGF and EGF, the maintenance medium will allow for the proliferation of ReNcell® immortalized VM and CX neural stem cells. Withdrawal of the growth factors from ReNcell® NSC Maintenance Medium will result in the spontaneous differentiation of ReNcell® immortalized neural progenitor cells |
| Materials Required but Not Delivered | 1. ReNcell® CX Immortalized Cell Line (CHEMICON Cat. No. SCC007) 2. ReNcell® VM Immortalized Cell Line (CHEMICON Cat. No. SCC008) 3. Basic fibroblast growth factor (bFGF; FGF-2; Specific Activity greater than 2 X 106 Units/mg. CHEMICON Cat. No. GF003) 4. Epidermal growth factor (EGF; Specific Activity greater than 1 x 107 Units/mg; CHEMICON Cat. No. GF001) 5. Laminin (Sigma Cat. No. L-2020) 5. Accutase (CHEMICON Cat. No. SCR005) 6. Tissue culture-ware 7. Phosphate-Buffered Saline (1X PBS) (CHEMICON Cat. No. BSS-1005-B) 8. Fixative (e.g. 4% Paraformaldehyde in 1X PBS) 9. Blocking Solution (5 percent normal donkey serum, 0.3 % Triton X-100 in 1X PBS) 10. Primary and secondary antibodies |
| Product Information | |
|---|---|
| Presentation | ReNcell® NSC Maintenance Medium contains DMEM/F12, HEPES, L-glutamine, human serum albumin, human transferrin, putrescine dihydrochloride, human recombinant insulin, L-thyroxine, Tri-iodo-thyronine, progesterone, sodium selenite, heparin, corticosterone. The media does not contain antibiotics. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | ReNcell® NSC Maintenance Medium is a defined serum-free, growth factor-free neural stem cell medium that has been optimized for the growth & in vitro differentiation of ReNcell® immortalized human neural progenitor cells. |
| Key Applications |
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| Biological Information | |
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| Media Form | Liquid |
| Stem Cell Type |
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| Product Usage Statements | |
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| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | SCM005, ReNcell® Neural Stem Cell Maintenance Medium should be stored at -20°C until ready to use. Upon thawing, the maintenance medium should be stored at 2-8°C and given a 1-month expiration dating. |
| Packaging Information | |
|---|---|
| Material Size | 500 mL |