Key Specifications Table
| Key Applications |
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| Cult |
| Description | |
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| Catalogue Number | SCM017 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | ENStem-A™ Neuronal Differentiation Medium |
| Overview | ENStem-A™ Neuronal Differentiation Medium is a specially formulated medium optimized for the preferential differentiation of ENStem-A™ human neural progenitor cells to a neuronal lineage. The medium has been extensively validated on ENStem-A™ Human Neural Progenitor Cells. Using ENStem-A™ Neuronal Differentiation Medium, relatively pure populations of neuronal cells (>90% bIII-tubulin positive) are obtained. Very low levels of astrocytes (<0.5% GFAP positive) are detected. Sufficient medium is supplied to provide for 100 separate reactions at 1 mL volume or 10 reactions at 10 mL volume. |
| Materials Required but Not Delivered | • L-Glutamine (Cat. No. TMS-002-C) • ENStem-A™ Human Neural Progenitor Cells Kit (Cat. No. SCR055) • ENStem-A™ Neural Expansion Medium (Cat. No. SCM004) • ENStem-A™ Neural Freezing Medium (Cat. No. SCM011) • Human Neural Stem Cell Characterization Kit (Cat. No. SCR060) • Poly-L-ornithine (Sigma Part No. P3655) • Laminin (Cat. No. CC095) • Penicillin/Streptomycin Solution (Cat. No. TMS-AB2-C) • Accutase™ (Cat. No. SCR005) • Chamber slides • Tissue culture-ware • Phosphate-Buffered Saline (1X PBS) (Cat. No. BSS-1005-B) • Hemocytometer • Microscope |
| Product Information | |
|---|---|
| Quality Level | MQ100 |
| Applications | |
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| Application | The ENStem-A Neuronal Differentiation Medium is a specially formulated medium optimized for the preferential differentiation of ENStem-A human neural progenitor cells to a neuronal lineage. |
| Key Applications |
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| Application Notes | Cell culture protocols for use with ENStem-A™ Human Neural Progenitor Cells are available in the datasheets that accompany the ENStem-A™ Human Neural Progenitor Expansion Kit (Part No. SCR055). |
| Biological Information | |
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| Media Form | Liquid |
| Stem Cell Type |
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| Product Usage Statements | |
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| Quality Assurance | Each lot of media is tested for the optimal differentiation of ENStem-A™ Human Neural Progenitor Cells to a neuronal lineage. Sterility Testing: Negative |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | ENStem-A™ Neuronal Differentiation Medium should be stored at -20°C until ready to use. Upon thawing, fresh L-Glutamine (not provided) should be added for a final concentration of 2 mm to the medium. Thawed medium should be stored at 2-8°C and given a 1-month expiration dating. Dispense into aliquots to avoid repeated heating prior to each use. |
| Packaging Information | |
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| Material Size | 100 mL |