| Description | |
|---|---|
| Catalogue Number | SCR005 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Accutase cell detachment solution |
| Overview | A cell detachment solution of proteolytic and collagenolytic enzymes. Useful for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware, including SmartPlastic™. ACCUTASE™ does not contain mammalian or bacterial derived products. Because the reagent does not contain universally proteolytic enzymes it may not release all cell/tissue types uniformly. Some optimization of cell type and passage number, as well as, incubation time and reagent strength typically needs to be performed to optimize the reagents effectiveness. ACCUTASE™ has been shown to be effective on a wide variety of cell types including: fibroblasts, keratinocytes, vascular endothelial cells, hepatocytes, vascular smooth muscle cells, hepatocyte progenitors, primary chick embryo neuronal cells, bone marrow stem cells, adherent CHO and BHK cells, macrophages, 293 cells, L929 cells, immortalized mouse testicular germ cells, 3T3, Vero, COS, HeLa, NT2, MG63, M24 and A375 metastatic melanoma, gliomas U251 and D54, HT1080 fibrosarcoma cells, and Sf9 insect cells. Each lot of ACCUTASE™ is tested for Sterility (by USP membrane filtration method), enzymatic activity (tested with synthetic chromagenic tetrapeptides) and cell detachment from tissue culture plastic. |
| Product Information | |
|---|---|
| Presentation | 100 ml, ready to use, frozen sterile filtered (0.2 micron) liquid. 1X ACCUTASE enzymes in Dulbeccos PBS (0.2 g/L KCl, 0.2 g/L KH2PO4, 8 g/L NaCl, and 1.15 g/L Na2HPO4) containing 0.5 mM EDTA·4Na and 3 mg/L Phenol Red. |
| Quality Level | MQ300 |
| Applications | |
|---|---|
| Application | A cell detachment solution of proteolytic & collagenolytic enzymes. The reagent is useful for creating single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue. |
| Application Notes | Cell Detachment 1. Thaw ACCUTASE™ at room temperature. 2. Wash plate, flask or beads with sterile PBS. 3. Add ACCUTASE™ to culture dish or flask using aseptic procedures at 10 ml per 75-cm2 surface area. 4. Return culture to 37°C incubator and allow cells to detach (5-10 minutes). 5. Count cells and passage as usual. No additional washes or enzyme inhibitors are required Optimal incubation times and reagent strength needs to be determined for each cell/tissue type tested by the enduser. |
| Biological Information | |
|---|---|
| Media Form | Liquid |
| Stem Cell Type |
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| Product Usage Statements | |
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| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Stable when stored at -20°C. Refer to lot expiration date. Recommended storage upon receipt is -20°C. After thawing, ACCUTASE may be stored for up to 2 months at 4°C. DO NOT STORE AT ROOM TEMP |
| Packaging Information | |
|---|---|
| Material Size | 100 mL |