Takara NucleoMag DNA FFPE \1 x 96 Preps

NucleoMag DNA FFPE kits employ superparamagnetic beads to enable high-throughput DNA purification from formalin-fixed, paraffin-embedded (FFPE) samples using manual or automated processing. The kits can be used to process fresh or archived samples and are compatible with inputs of ≤5 mg of tissue and ≤15 mg of paraffin. Purified DNA fragments typically range in size from 50 bp–5 kbp and are eluted in volumes >25 µl. The kits include blue-colored Paraffin Dissolver for convenient paraffin removal and enable processing of 96 samples in ~2 hours.

Cat. # 744320.1 includes sufficient reagents and materials for processing of 96 samples.

NucleoMag DNA FFPE provides better PCR performance than competitor kits.Genomic DNA was purified from FFPE samples using NucleoMag DNA FFPE in comparison with kits from Competitor A and Competitor I and analyzed using a LightCycler PCR system and the DyNAmo Capillary SYBR Green qPCR Kit (amplicon size: 191 bp). qPCR of DNA purified with kits from Competitor A (Panel A) and Competitor I (Panel B) resulted in higher Ct values, indicating lower DNA yields compared to samples purified with NucleoMag DNA FFPE.

The NucleoMag DNA FFPE procedure.

NucleoMag DNA FFPE provides higher yields than competitor kits.Genomic DNA was purified in triplicate from embedded mouse lung, liver, and kidney tissue sections using NucleoMag DNA FFPE and two competitor kits. The NucleoMag DNA FFPE kit provided higher DNA yields than the competitor kits (A, I).