Takara PrimeScript IV 1st strand cDNA Synthesis Mix \50 Rxns

PrimeScript IV 1st strand cDNA Synthesis Mix contains a 5X premix for synthesizing first-strand cDNA from total RNA or poly A+ RNA. A cDNA synthesis reaction can be started simply by adding template RNA and water to the premix, which already contains PrimeScript IV Reverse Transcriptase, RNase Inhibitor, Oligo dT Primer, dNTP Mixture, and reaction buffer. The premix also contains an accessory protein that eliminates the need for RNA denaturation, which is commonly performed before reverse transcription.

Yield of dystrophin cDNA product (6,930 bp) with PrimeScript IV.

Synthesis of cDNA with better sensitivity and specificity compared to competitor’s products. PrimeScript IV resulted in high fidelity synthesis of a 6,930 bp region of the dystrophin gene from just 100 ng of template.

PrimeScript IV gave higher yields of cDNA without RNA denaturation compared to Competitor Tv4.