Takara NucleoBond Xtra BAC \25 Preps

NucleoBond Xtra BAC (25) 25 preps for the isolation of low-copy larg construct plasmid DNA - NucleoBond Xtra BAC Columns, buffers, RNase A

NucleoBond Xtra BAC is suitable for restriction digestion: BAC DNA (300 kb) was isolated in triplicate from 500 mlE. coliDH5a using NucleoBond Xtra BAC and competitor products (Q and I).After precipitation, BAC DNA was reconstituted in 1,000 μl TE buffer and 5 μl of each sample was used for analysis on a 1% TAE agarose gel. Obtained yields: MN: 150 μg, Q: 44 μg, I: 75 μg. Restriction digestion of BAC DNA isolated with NucleoBond Xtra BAC. BAC DNA samples were purified from overnight cultures ofE. coliDH5a transformed with a pBAC10 clone. Approximately 3 μg of DNA from each sample was digested with 3 units of MspI, HindIII, or EcoRI at 37°C for 2 hr. Digestions were analyzed on a 1% TAE agarose gel. Lane 1: undigested BAC DNA, lane 2: MspI-digested, lane 3: HindIII-digested, lane 4: EcoRI-digested, lane m: marker.

High selectivity and specific characteristics of the optimal silica matrix make this anion exchanger ideal for the purification of very large constructs, such as P1, BACs, and PACs.Using the NucleoBond Xtra BAC kit, it takes just 75 min to isolate, for example, BAC DNA free from any kind of RNA, proteins, metabolites, dyes, and carbohydrates. Low amounts of impurities, which often act as strong inhibitors for enzymatic processing, transfections, transcriptions, etc., are completely removed.