Takara Capturem His-Tagged Purification Maxiprep Kit \6 Purifications

The Capturem His-Tagged Purification Maxiprep Kit provides single-use disposable membrane spin columns for simple, rapid purification of his-tagged proteins in up to 23 mL of clarified lysate. The columns are suitable for use under native or denaturing conditions, and in the presence of additives such as DTT (up to 10 mm), βME (up to 30 mm), TCEP (up to 5 mm), EDTA (up to 10 mm), or glycerol. Each column requires a minimum elution volume of 500 µl.

Purification workflow for Capturem His-Tagged Purification 96-Well Plate.The his-tagged protein of interest is first bound to the membrane, and then washed and eluted with the appropriate buffers. Note: protocol time varies for each format. Check user manuals for specifics on protocol times.

Available Capturem spin column and filtration device formats.Pictured from left to right: nanoprep, miniprep, 96-well, 24-well, maxiprep, and large-volume Capturem formats. Formats available vary for the different functionalized membranes. Check product pages for list of available formats.

Load volumes and yields of Capturem purification formats.