Takara Capturem His-Tagged Purification 96-Well Plate \1 x 96-well plate

Capturem His-Tagged Purification 96 is a single-use, disposable 96-well plate for simple, rapid purification of his-tagged proteins from up to 1 mL of clarified lysate per well. This plate is suitable for use under native or denaturing conditions, in the presence of additives such as DTT (up to 10 mm), βME (up to 30 mm), TCEP (up to 5 mm), EDTA (up to 10 mm), or glycerol. Capturem His-Tagged Purification 96 is compatible with automated liquid handlers equipped with vacuum filtration or centrifuges equipped with appropriate plate holders. Each well requires a minimum elution volume of 100 µl.

Purification workflow for Capturem His-Tagged Purification 96-Well Plate.The his-tagged protein of interest is first bound to the membrane, and then washed and eluted with the appropriate buffers. Note: protocol time varies for each format. Check user manuals for specifics on protocol times.

Available Capturem spin column and filtration device formats.Pictured from left to right: nanoprep, miniprep, 96-well, 24-well, maxiprep, and large-volume Capturem formats. Formats available vary for the different functionalized membranes. Check product pages for list of available formats.

Load volumes and yields of Capturem purification formats.