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Millipore Anti-Monomethyl-Histone H3 (Lys36) Antibody Is A Rabbit Polyclonal Antibody For Detection Of Histone H3 Monomethylated At Lysine 36. This Purified Polyclonal Antibody Also - Mill (Additional S&H or Hazmat Fees May Apply)
Histone H3 is one of four histones that form a core octameric structure that is required for the packaging of DNA into nucleosomes, the most basic level of chromatin arrangement. Histones undergo a number of post-translational modifications (PTM) at their N- and C-terminal tails which can alter chromatin structure and stability, and influence DNA repair and gene expression. Histone H3 undergoes methylation of its lysine and arginine residues, with varying biological outcomes. The lysine 36 residue undergoes mono-, di-, or trimethylation which may be performed by one of many methyltransferase enzymes (SET2, NSD1, NSD2, NSD3, ASH1, SMYD2, SETMAR, KMT3A, and SETD3). Several studies have indicated that methylated lysine 36 residues promote Rpd3S-mediated deacetylation of other histones resulting in gene activation.
Product Information
Format
Affinity Purified
Control
HeLa acid extract and various recombinant Histone proteins
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-monomethyl-Histone H3 (Lys36) Antibody is a rabbit polyclonal antibody for detection of Histone H3 monomethylated at lysine 36. This purified polyclonal antibody also known as Anti-H3K36me1, has dot blot (DB) proven specificity & has been validated in WB, IP.
Key Applications
Western Blotting
Immunoprecipitation
Dot Blot
Application Notes
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated monomethyl-Histone H3 (Lys36) from HeLa acid extract.
Dot Blot Specificity Analysis: Unmodified and various modified Histone peptides (see table) were probed with Anti-monomethyl-Histone H3 (Lys36) (0.5 µg/mL).
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to the N-terminus of human Histone H3 monomethylated at Lys36.
Epitope
Monomethylated Lys36
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Specificity
This antibody recognizes the N-terminus of Histone H3 monomethylated at Lys36.
Species Reactivity
Mouse
Human
Rat
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_003484
Gene Symbol
HIST1H3A
H3FA
HIST1H3B
H3FL
HIST1H3C
H3FC
HIST1H3D
H3FB
HIST1H3E
H3FD
HIST1H3F
H3FI
HIST1H3G
H3FH
HIST1H3H
H3FK
HIST1H3I
H3FF
HIST1H3J
H3FJ
HIST3H3
H3FT
H3T
MGC126886
Purification Method
Affinity Purfied
UniProt Number
Q16695
Molecular Weight
~17 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in HeLa acid extract and various recombinant Histone proteins.
Western Blot Analysis: 0.05 µg/mL of this antibody detected monomethyl-Histone H3 (Lys36) in 10 µg of HeLa acid extract.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
