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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H | WB, FC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 04-147 |
| Description | Anti-ASC Antibody, clone 2EI-7 |
| Alternate Names |
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| Background Information | Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD containing cell death regulators include RAIDD, RICK, Bcl10, Apaf-1, ARC, caspase-2 and caspase-9. A novel CARD domain containing protein has been identified in human and mouse and designated ASC and TMS1. Irregular expression of ASC/TMS1 induced apoptosis through activation of caspase-9 and inhibited the survival of human breast cancer cells. Overexpression of ASC/TMS1 induced DNA fragmentation. ASC/TMS1 is expressed in a variety of human and mouse tissues. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mm NaCl) with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect ASC using this Anti-ASC Antibody, clone 2EI-7 validated for use in WB, FC. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | Full length recombinant human ASC. |
| Epitope | Unknown |
| Clone | 2EI-7 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | This antibody recognizes ASC. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Tested on mouse. Predicted to react with human based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. |
| Gene Symbol |
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| Purification Method | Protein g Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: Promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase 9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases. SIZE: 195 amino acids; 21627 Da SUBUNIT: Forms complexes with other DAPIN domain-containing proteins. Interacts with CIAS1/PYPAF1 and PYDC1. SUBCELLULAR LOCATION: Cytoplasm. Note=Upstream of caspase activation, a redistribution from the cytoplasm to the aggregates occurs. These appear as hollow, perinuclear spherical, ball-like structures. TISSUE SPECIFICITY: Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U937, but not in Jurkat T- cell lymphoma and Daudi Burkitts lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and Molt 4 lymphocytic leukemia cells.DOMAIN:SwissProt: Q9ULZ3 Interacts with CIAS1/PYPAF1 and PYDC1 via the DAPIN domain. PTM: Phosphorylated. SIMILARITY: Contains 1 CARD domain. & Contains 1 DAPIN domain. MISCELLANEOUS: In breast tumorigenesis, methylation-mediated silencing may affect genes and proteins that act as positive mediators of cell death. |
| Molecular Weight | ~22 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot on mouse spleen tissue lysate. Western Blot Analysis: 0.5 µg/mL of this antibody detected ASC in mouse spleen tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |