Key Specifications Table
| Key Applications | Species | Source | Purity |
|---|---|---|---|
| CULT | Human | CHO cells (Chinese Hamster Ovarian cells) | Greater than 98% by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per μg (1EU/ μg) of IL-12. |
| Description | |
|---|---|
| Catalogue Number | IL029 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Interleukin-12 Protein, Recombinant human |
| Overview | Interleukin-12 (IL-12) is a potent regulator of cell mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes, and connective tissue type mast cells. Among its biological activities, IL-12 promotes the growth and activity of activated NK, CD4+ and CD8+ cells and induces the development of IFN-gamma producing Th1 cells. Human IL-12 is a disulfide linked heterodimeric protein with a molecular weight of 75 kDa comprised of disulfide-bonded 35 kDa (p35) and 40 kDa (p40) subunits. |
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| Product Information | |
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| Presentation | The sterile filtered solution was lyophilized from 0.5X PBS pH 7.2 |
| Quality Level | MQ100 |
| Applications | |
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| Application | The recombinant human Interleukin-12 protein (or IL-12 protein) is a potent regulator of cell mediated immune responses & it induces IFN-gamma production by NK & T cells. |
| Key Applications |
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| Application Notes | For most in vitro applications, IL-12 exerts its biological activity in the concentration range of 1.0 to 10.0 ng/mL. |
| Biological Information | |
|---|---|
| Purity | Greater than 98% by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per μg (1EU/ μg) of IL-12. |
| Source | CHO cells (Chinese Hamster Ovarian cells) |
| Specific Activity | The ED50 as determined by the induction of IFN-g production from NK cells co-stimulated with IL-18 was <= 1.0 ng/mL, corresponding to a specific activity of >= 1.0 x 10E6 units/mg. |
| Entrez Gene Summary | This gene encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children. |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P29460 # Associates with IL23A to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak- Stat signaling cascade, stimulates memory rather than naive T- cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis. SIZE: 328 amino acids; 37169 Da SUBUNIT: Heterodimer with IL12A; disulfide-linked. The heterodimer is known as interleukin IL-12. Heterodimer with IL23A; disulfide- linked. The heterodimer is known as interleukin IL-23. Also secreted as a monomer. SUBCELLULAR LOCATION: Secreted. PTM: Known to be C-mannosylated in the recombinant protein; it is not yet known for sure if the wild-type protein is also modified. DISEASE: SwissProt: P29460 # Defects in IL12B are a cause of mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity, whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance. SIMILARITY: SwissProt: P29460 ## Belongs to the type I cytokine receptor family. Type 3 subfamily. & Contains 1 fibronectin type-III domain. & Contains 1 Ig-like C2-type (immunoglobulin-like) domain. |
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| Product Usage Statements | |
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| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | The lyophilized IL-12, though stable at room temperature, is best stored desiccated below 0°C. Reconstitute with sterile water to a concentration of 0.2 mg/mL. Reconstituted IL-12 should be stored in undiluted aliquots at -20°C for up to 6 months. |
| Packaging Information | |
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| Material Size | 10 µg |