GenScript Gencrispr™ Cas12a (Cpf1) Nuclease; 50 μg

Theclustered regularly interspaced short palindromic repeats, known as CRISPR systems are adaptive immune mechanisms commonlypresent in archaea and bacteria. The CRISPR systems enable the host to specificallytarget and cleave foreign nucleic acids thus targeting infectiousviruses andplasmids. Recently, a type V CRISPR system has been identified in severalbacteria, the Cpf1 CRISPR from Prevotella and Francisella 1. In contrast toCas9 systems, CRISPR/Cpf1 systems are smaller in size, do not require anadditional trans-activating crRNA (tracrRNA), and allow for targeting ofadditional genomic regions by cleaveing the target DNA proceeded by a shortT-rich protospacer-adjacent motif (PAM). On the other hand, the Cas9 system requiresa G-rich PAM following the target DNA. Furthermore, Cas12a/Cpf1 introduces astaggered DNA double stranded break with a 4 or 5-nt 5’ overhang. RecombinantAcidaminococcus sp. BV3L6 Cas12a (cpf1) nuclease is expressed in E. coli andpurified. The nuclease contains nuclear localization sequence (NLS) at theC-terminus and 6× His-tag at the C-terminus.