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GenScript Gencrispr™Ultra Nls-Cas9-Basic Gmp; 1mg
List Price $1,695.00 Your Price $1,695.00
GenScript Gencrispr™Ultra Nls-Cas9-Basic Gmp - GSCRPT (Additional S&H or Hazmat Fees May Apply)
NETA PART: GSCRPT-Z03623-1
MFG.PART: Z03623-1
UNSPSC: 12352204
Manufacturer: GenScript USA Inc
Overview
| Description | GenCRISPR™Ultra NLS-Cas9-basic GMP is utilized for CRISPR gene editing applications. The Cas9nuclease forms a stable ribonucleoprotein (RNP) complex with the guide RNA(gRNA) component. With the help of nuclear localization signal (NLS) expressedwith the Cas9 nuclease, the RNP complex enters the nucleus and cleaves thetarget gene. When compared with a plasmid-based delivery system, the RNPdelivery system has been observed to increase the on-target gene editingefficiency and decrease off-target effects. | ||||||
| Selection Guide | GenScriptprovides two types of Cas9 nucleases in basic GMP* for selection based on yourspecific downstream applications.
|
Applications
| sgRNA-dependentdouble-stranded DNA cleavage. |
Properties
| Source | Recombinant Cas9 nuclease (wt) with anN-terminal NLS expressed in E.coli |
| Species | S. pyogenes |
| Tag | Tag-free |
| Theoretical Molecular Weight | 160 kDa |
| Concentration | 10mg/mL |
| Active temperature | Optimal at 37 °C. |
| Storage Buffer | 25 mM Tris-HCl, 300 mM NaCl, 0.1 mM EDTA, 50%Glycerol pH 8.0. |
| Storage & Stability | Storeat -20 °C for up to 12 months from the date ofmanufacture.Avoid repeated freeze-thaw cycles. Do not store below -20 °C! |
Quality Control Specifications
| Appearance | Clear, colorless, liquid |
| Purity | ≥ 95% as analyzed by SDS-PAGE ≥ 95% as analyzed by SEC-HPLC |
| Concentration by A280 | 10 mg/ml ± 1 mg/ml |
| Bioactivity (in vitro) | ≥ 90% |
| Residual DNase | ≤ 10 ng/mg |
| Residual RNase | ≤ 1 ng/mg |
| Endotoxin Level | ≤ 10 EU/mg |
| Residual HCP | ≤ 10 ng/mg |
| Residual HCD | ≤ 1 ng/mg |
| Bioburden | < 1 CFU/ml |
| Mycoplasma | < LOD |
Background
| References | 1. Jinek, Martin, et al. "A programmable dual-RNA–guidedDNA endonuclease in adaptive bacterial immunity."science337.6096(2012): 816-821. 2. Chen, Sean, et al. "Highly efficient mouse genomeediting by CRISPR ribonucleoprotein electroporation of zygotes."Journalof Biological Chemistry291.28 (2016): 14457-14467. 3. Larson, Matthew H., et al. "CRISPR interference(CRISPRi) for sequence-specific control of gene expression."Natureprotocols8.11 (2013): 2180-2196. 4. Ran, F. Ann, et al. "Genome engineering using theCRISPR-Cas9 system."Nature protocols8.11 (2013):2281-2308. 5. Kim, Sojung, et al. "Highly efficient RNA-guided genomeediting in human cells via delivery of purified Cas9ribonucleoproteins."Genome research24.6 (2014):1012-1019. |
| Annotation | *basic GMP is a branding term that GenScript uses todescribe reagents manufactured in compliance with ISO 9001 and ISO 13485quality management system standards and with more stringent process controlsand relatively complete documentation records. GenScript is capable ofproviding documents, site audit and other support to help with theapplications of your projects in specificregions. |
For research or manufacturinguse. Not intended for clinical use. Direct human use, including taking orallyand injection are forbidden.
| SKU | GSCRPT-Z03623-1 |
|---|---|
| Supplier Part Number | Z03623-1 |
| UM | EA |
| UNSPSC | 12352204 |
| Manufacturer | GenScript USA Inc |
| Temperature | Storeat -20 °C for up to 12 months from the date ofmanufacture.Avoid repeated freeze-thaw cycles. Do not store below -20 °C! |
| CountryOfOrigin | United States |
| ProductLine | GSCRPT |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 7 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |