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GenScript Gencrispr™Ultra Espcas9-2nls-Research; 0.5mg
List Price $765.00 Your Price $765.00
GenScript Gencrispr™Ultra Espcas9-2nls-Research - GSCRPT (Additional S&H or Hazmat Fees May Apply)
NETA PART: GSCRPT-Z03622-0.5
MFG.PART: Z03622-0.5
UNSPSC: 12352204
Manufacturer: GenScript USA Inc
Description
The GenCRISPR™Ultra eSpCas9 product line provides customers with a selection of research use,basic GMP and GMP compliant Cas9 nucleases. The Cas9 protein can be formed withthe guide RNA into a ribonucleoprotien (RNP) complex. The use of an RNP complexto perform gene editing has been shown to reduce the challenges encounteredwith other CRISPR gene editing techniques such as viral and plasmid delivery.Challenges include off-target effects, cell viability and transcription/translationalchallenges. GenCRISPR™Ultra eSpCas9-2NLS is a mutant form of Cas9 nuclease and is produced byexpression in an E. coli straincarrying a plasmid encoding the eSpCas9 gene from Streptococcus pyogenes with double-ended nuclear localizationsignal (NLS). GenCRISPR™ Ultra eSpCas9-2NLS delivers higher fidelity and lessoff-target activity than wild-type SpCas9 nuclease. |
Note
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
Applications
| gRNA-dependent double-stranded DNA cleavage |
Properties
| Expression System | RecombinantCas9 with double-ended NLS expressed by E.coli |
| Species | S. pyogenes |
| Tag | Tag-free |
| Molecular Weight | ~160kDa |
| Concentration | 10mg/mL |
| Active temperature | ThiseSpCas9 is active at 37°C. |
| Formulation | Suppliedas a solution of 25 mM Tris, 300 mM NaCl,0.1 mM EDTA, 50% Glycerol pH 8.0 at 25°C. |
| Storage & Stability | This product remains stable up to 12 months at -20°C. Avoid repeated freeze-thaw cycles. |
| Key Features | High knockoutefficiencies: Consistent high performance in in-vitro plasmid cleavage test. Tag-free: No extra tagamino acid Animal-free: Plant-based culture media, Enzyme-freechromatographic purification. DNA-free: Noexternal DNA added to the system. |
Quality Control Specifications
| Appearance | Clear, colorless liquid |
| Purity | ≥ 95% as analyzed by SDS-PAGE ≥ 90% as analyzed by SEC-HPLC |
| Concentration by A280 | 10 mg/ml±1 mg/ml |
| Bioactivity (in vitro) | ≥ 90% |
| Residual DNase | Non-specific DNase activity |
| Residual RNase | Non-specific RNase activity |
| Endotoxin Level | ≤ 10 EU/mg as analyzed by gel clotting method |
| SKU | GSCRPT-Z03622-0.5 |
|---|---|
| Supplier Part Number | Z03622-0.5 |
| UM | EA |
| UNSPSC | 12352204 |
| Manufacturer | GenScript USA Inc |
| Temperature | This product remains stable up to 12 months at -20°C. Avoid repeated freeze-thaw cycles. |
| ProductLine | GSCRPT |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 7 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |