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GenScript Benz-Neburase™, Tag-Free; 10kU
List Price $49.00 Your Price $49.00
GenScript Benz-Neburase™, Tag-Free - GSCRPT (Additional S&H or Hazmat Fees May Apply)
NETA PART: GSCRPT-Z03695-10
MFG.PART: Z03695-10
UNSPSC: 12352204
Manufacturer: GenScript USA Inc
Properties
| Expression System | E.coli |
| Species | Serratia Marcescen |
| Tag | Tag-free |
| Theoretical Molecular Weight | 27.5 kDa |
| Biological Activity | ≥ 1.1×106U/mg One unitof Benz-Neburase, tag-free is defined as the amount of enzyme for a ∆A260 of1.0 (equivalent to the complete digestion of 37μg DNA) in 30 min. |
| Formulation | Suppliedas a solution of 20 mM Tris-HCl, 2 mM MgCl2, 20 mM NaCl, 50% Glycerol, pH 8.0. |
| Apparent Molecular Weight | ~27.5 kDa, on SDS-PAGEunder reducing conditions |
| Storage & Stability | This product remains stable for up to 2 weeks at 4 °C or up to 24 months at -20 °C. Avoid repeated freeze-thaw cycles. Do not store below -20 °C! |
Applications
| The Benz-Neburase™is an endonuclease capable ofremoving all forms of DNA and RNA, including double stranded, single stranded,linearized, and circular forms. The Benz-Neburase iscommonly used in biopharmaceutical production such as vaccine, viral vector,gene and cell therapy manufacturing facilities. | |
| The activity of Benz-Neburase™, tag-free requires 1-2 mM Mg2+. |
Quality Control Specifications
| Purity | ≥ 95% as analyzed by reducing SDS-PAGE |
| Enzyme Activity | ≥250 U/μL |
| Endotoxin Level | ≤ 0.1 EU/kU as determined by gel clotting method. |
Background
| Target Background | The Benz-Neburase is agenetically engineered endonuclease from Serratia marcescens used as aDNA eraser in the purification processes of biological molecules. The enzymecleaves all forms of DNA and RNA into smaller nucleotides of around 5-8 basepairs. Benz-Neburase requires divalent cation, preferably Mg2+ foractivity, displays a broad pH tolerance, ranging from pH 6 to pH 10, with an optimalpH of 8-8.5, and has a wide temperature tolerance, ranging from 35 °C to 44 °C. The nuclease is aphysiologic homodimer and functions more progressively than the monomer. Twodisulfide bonds in the nuclease are crucial to its activity and stability. Theenzyme is active in a broad range of conditions and is free of proteolyticactivity. This makes the enzyme especially useful for biopharmaceutical applicationswith contaminating DNA residue, such as lysed host cells in viral vectormanufacturing processes. |
| Synonyms | alternative to Benzonase® Benzonaseis a registered trademark of Merck KGaA |
| References | 1. Nestle, Marion, and W. K. Roberts. "Anextracellular nuclease from Serratia marcescens: I. Purification and someproperties of the enzyme."Journal of Biological Chemistry244.19(1969): 5213-5218. 2. Benedik, Michael J., and Ulrich Strych."Serratia marcescens and its extracellular nuclease."FEMSmicrobiology letters165.1 (1998): 1-13. 3. Filimonova, Maria N., Kurt L. Krause, andMichael J. Benedik. "Kinetic studies of the Serratia marcescensextracellular nuclease isoforms."Biochemistry and molecularbiology international33.6 (1994): 122-1032. 4. Friedhoff, Peter, et al. "A procedure forrenaturation and purification of the extracellular Serratia marcescens nucleasefrom genetically engineered Escherichia coli."Protein expressionand purification5.1 (1994): 37-43. 5. Franke, Ingo, GregorMeiss, and Alfred Pingoud. "On the Advantage of Being a Dimer, a CaseStudy Using the Dimeric Serratia Nuclease and the Monomeric Nuclease fromAnabaena sp. Strain PCC 7120."Journal of Biological Chemistry274.2(1999): 825-832. |
For laboratory research use only. Direct human use,including taking orally and injection and clinical use are forbidden.
| SKU | GSCRPT-Z03695-10 |
|---|---|
| Supplier Part Number | Z03695-10 |
| UM | EA |
| UNSPSC | 12352204 |
| Manufacturer | GenScript USA Inc |
| Temperature | This product remains stable for up to 2 weeks at 4 °C or up to 24 months at -20 °C. Avoid repeated freeze-thaw cycles. Do not store below -20 °C! |
| ProductLine | GSCRPT |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 7 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |