| Description | |
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| Catalogue Number | EMS0008 |
| Description | LysargiNase |
| Alternate Names |
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| Background Information | LysargiNase is a metalloproteinase found in the thermophilic archaea Methanosarcina acetivorans. It specifically cleaves before lysine and arginine residues in proteins. This cleavage results in peptides with molecular weights similar to tryptic peptides, but with N-terminal lysine or arginine residues that can be fragmented with b ion-dominated spectra. This can improve protein C-terminal peptide identification and several arginine rich phosphosite assignments. Unlike with trypsin, LysargiNase cleavage also occurs at methylated lysine and arginine, allowing detection of these epigenetic modifications. References 1)Tallant C, García-Castellanos R, Seco J, Bauman U, Gomis-Rüth FX (2006) Molecular analysis of ulilysin, the structural prototype of a new family of metzincin metalloproteases. J Biol Chem. 281(26):17920-17928 2)Huesgen PF, Lange PF, Rogers LD, Solis N, Eckhard U, Kleifeld O, Goulas T, Gomis-Rüth FX, Overall CM (2015) LysargiNase mirrors trypsin for protein C-terminal and methylation-site identification. Nat Methods 12(1):55-58 3)Tsiatsiani L, Heck AJR (2015) FEBS Journal 282: 2612-2626 |
| Product Information | |
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| Components |
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| Presentation | Each vial contains 20 µg LysargiNase, prepared by recombinant expression in E. coli and lyophilized from buffer. Upon reconstitution in 20 µL water, the enzyme is in 50 mM HEPES, 5 mM CaCl2, pH 7.5. |
| Quality Level | MQ100 |
| Applications | |
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| Application | LysargiNase is suitable for peptide or protein digestion in solution or in gel. |
| Application Notes | LysargiNase is suitable for peptide or protein digestion in solution or in gel. It is active under a variety of solvent conditions including 5 mM TCEP, 5% methanol, 5% acetonitrile, 0.8 M urea, 0.1% RapiGest, 1% deoxycholate, 0.2% SDS and 1% NP-40. It is active up to 55°C at pH 6 – 9. LysargiNase is reversibly inhibited by 1, 10-phenanthroline, EDTA and other calcium and zinc chelating agents. For peptide or protein digestion, a ratio of between 1:100 to 1:20 (w/w) enzyme:substrate is recommended. |
| Biological Information | |
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| Concentration | Please refer to lot specific datasheet. |
| Product Usage Statements | |
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| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Store the lyophilized product at -20°C for up to 2 years. After reconstitution, the unused material can be stored in the freezer in aliquots. Avoid repeated freeze thaw cycles. |
| Packaging Information | |
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| Material Size | 1 |