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LysargiNase mirrors trypsin for protein C-terminal and methylation-site identification
Background Information
LysargiNase is a metalloproteinase found in the thermophilic archaea Methanosarcina acetivorans. It specifically cleaves before lysine and arginine residues in proteins. This cleavage results in peptides with molecular weights similar to tryptic peptides, but with N-terminal lysine or arginine residues that can be fragmented with b ion-dominated spectra. This can improve protein C-terminal peptide identification and several arginine rich phosphosite assignments. Unlike with trypsin, LysargiNase cleavage also occurs at methylated lysine and arginine, allowing detection of these epigenetic modifications.
References 1)Tallant C, García-Castellanos R, Seco J, Bauman U, Gomis-Rüth FX (2006) Molecular analysis of ulilysin, the structural prototype of a new family of metzincin metalloproteases. J Biol Chem. 281(26):17920-17928 2)Huesgen PF, Lange PF, Rogers LD, Solis N, Eckhard U, Kleifeld O, Goulas T, Gomis-Rüth FX, Overall CM (2015) LysargiNase mirrors trypsin for protein C-terminal and methylation-site identification. Nat Methods 12(1):55-58 3)Tsiatsiani L, Heck AJR (2015) FEBS Journal 282: 2612-2626
Product Information
Components
Each vial contains 20 µg LysargiNase, prepared by recombinant expression in E. coli and lyophilized from buffer.
Presentation
Each vial contains 20 µg LysargiNase, prepared by recombinant expression in E. coli and lyophilized from buffer. Upon reconstitution in 20 µL water, the enzyme is in 50 mM HEPES, 5 mM CaCl2, pH 7.5.
Quality Level
MQ100
Applications
Application
LysargiNase is suitable for peptide or protein digestion in solution or in gel.
Application Notes
LysargiNase is suitable for peptide or protein digestion in solution or in gel. It is active under a variety of solvent conditions including 5 mM TCEP, 5% methanol, 5% acetonitrile, 0.8 M urea, 0.1% RapiGest, 1% deoxycholate, 0.2% SDS and 1% NP-40. It is active up to 55°C at pH 6 – 9. LysargiNase is reversibly inhibited by 1, 10-phenanthroline, EDTA and other calcium and zinc chelating agents. For peptide or protein digestion, a ratio of between 1:100 to 1:20 (w/w) enzyme:substrate is recommended.
Biological Information
Concentration
Please refer to lot specific datasheet.
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Store the lyophilized product at -20°C for up to 2 years. After reconstitution, the unused material can be stored in the freezer in aliquots. Avoid repeated freeze thaw cycles.
