AG Scientific Beta-Lactamase, 5 vials

Description:

Beta-lactamasesare enzymesproduced by bacteria (Also known as Penicillinase) that provideresistancetoβ-lactam antibioticssuch as penicillins, cephamycins, andcarbapenems(ertapenem), although carbapenemsare relatively resistant to beta-lactamase.

Beta-lactamase provides antibiotic resistance by breaking the antibiotics structure.Beta-Lactamase ensures sterility of bacterial infections by eliminating false negatives via the removal of antibiotics in blood samples.

These antibiotics all have a common element in their molecular structure: a four-atom ring known as aβ-lactam. Throughhydrolysis, the lactamase enzyme breaks the β-lactam ring open, deactivating the molecules antibacterial properties. Beta-lactam antibiotics are typically used to treat a broad spectrum ofGram-positiveandGram-negativebacteria. Beta-lactamases produced by Gram-negative organisms are usually secreted, especially when antibiotics are present in the environment.

Applications:

• Testing sterility of blood cultures - Blood cultures are routinely prepared in order to test for bacterial infection. False negative results might be obtained where the blood sample contains antibiotics. Incorporation of β-Lactamase in the culture medium will overcome this problem when cephalosporins/penicillins are present
• Testing for contamination of drugs by antibiotics - US Code of Federal regulations states that “If a reasonable possibility exists that a non-penicillin drug product has been exposed to cross-contamination with penicillin, the non-penicillin drug product shall be tested for the presence of penicillin” (21 CFR 211.176, Penicillin Contamination, FDA, BY-Lines No. 8 November 1977)
• Environmental monitoring of antibiotic manufacturing area
• Sterility testing of bulk antibiotics as described by US Pharmcopeia (USP) Chapter 71 and EP Section 2.6

Specifications:

• Sterile, gamma-radiated, pre-dispensed, vacuum-sealed vials (in packs of 10) for speed and ease-of-use
• >50 units/vial (at pH 7.0, 25°C)
• Bulk powder is not sterile, but has been filtered through a 0.45μm filter prior to freeze drying.

Additional Reading:

• Beta-Lactamase and Microbial Antibiotic Resistance
• Top 7 FAQs About Beta-Lactamase
• Beta-Lactamase Composition, Sterility & Stability
• Beta-Lactamase Inhibitors in Anti-Seizure Research