AG Scientific Pyruvate Oxidase, 5 KU

Description:

Pyruvate oxidase (E.C. 1.2.3.3) is originated from Aerococcus viridans and used as a component of screen-printed phosphate biosensor for monitoring phosphate concentrations in a sequencing batch reactor (SBR) system. It consumes phosphate in the presence of pyruvate and oxygen and generates hydrogen peroxide, carbon dioxide and acetylphosphate.

The enzyme is a flavoprotein with the relatively tightly bound cofactors flavin adenine dinucleotide, thiamine pyrophosphate, and a divalent metal ion, with Mn2+ being the most effective.
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Activity: 40 units/mg powder. One unit is defined as amount of enzyme required to generate 1umol of H202 per minute at 37C, pH 6.5. Contaminate info: Glutamate oxalacetate transaminase (GOT) 002% by activity; Glutamate pyruvate transaminase (GPT) 006% by activity; Lactate Oxidase 002% by activity; Catalase not detectable.

Applications:

• Study of aerobic metabolism of bacteria
• Used for enzymatic determination of AST and ALT
• Used to monitor phosphate concentration in SBR system

Additional Reading:

• Electrochemical Processes for Treating Water and Why it Matters
• Single-Use Biosensors for the Detection of Biomarkers
• Advances in Lab-on-a-Chip Technology