Corning Collagen I, Bovine, 30 mg

(This is the suggested replacement for 354257)

Corning® Collagen I, bovine, 30mg, is used either as a thin layer on tissue-culture surfaces to enhance cell attachment and proliferation, or as a gel to promote expression of cell-specific morphology and function. (Same as Cat. #40231)

Source

Bovine dermis

Quality

• Purity > 95% by SDS-PAGE
• Shown to promote attachment and spreading of HT-1080 human fibrosarcoma cells
• Tested for formation of a firm gel by exposure to ammonia vapors from solutions of 0.5 mg/ mL. Further dilution will decrease the rigidity of the gel. NOTE: This preparation contains native collagen molecules with a small amount of nicked or shortened sequences due to pepsin treatment.
• Tested and found negative for bacteria, fungi, and mycoplasma

Formulation

Liquid in 0.012 N HCl

Preparation and Storage

Stable for at least three months at 2° to 8°C. Do not freeze.

Handling

Effective as a gel or thin coating at a recommended concentration of 5-10 µg/cm² of growth surface depending on cell type