| Description | |
|---|---|
| Catalogue Number | IEVH85R |
| Trade Name |
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| Description | Immobilon®-E PVDF Membrane |
| Overview | Immobilon-E is the first PVDF membrane for Western blotting that can be wetted with with aqueous transfer buffer. This eliminates the alcohol pre-wet step required with other PVDF membranes. |
| Background Information | This 8.5cm x 10m roll offers 1-cut convenience for standard mini-blots (7 x 8.5cm) and midi-blots (8.5 x 13.5cm). It works with the optional Immobilon NOW Dispenser (catalog number IMDISP). Immobilon-E Membrane: - the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step. - 45 µM pore size recommended for most Western blotting applications, especially proteins greater than 20 kDa - Compatible with chemiluminescent and chromogenic detection Immobilon® PVDF membranes are offered in four types, each optimized for a different protein blotting application. Convenient blotting sandwiches feature pre-cut sheets of membrane and blotting filter paper. Immobilon® PVDF membranes have high protein adsorption, SO you won’t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes. Features & Benefits: - High protein binding provides stronger signal - Won’t crack, curl or fracture when cut - Low background - Superior staining capabilities - Can be stripped and reprobed multiple times |
| Product Information | |
|---|---|
| Detection method | ChemiluminescenceChromogenicRadioactive |
| Compatible Stains |
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| Filter Code | IEVH |
| Filter Color | White |
| Applications | |
|---|---|
| Application | The first PVDF transfer Membrane for western blotting that wets out in transfer buffer or water. For chemiluminescence or chromogenic detection. |
| Key Applications |
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| Biological Information | |
|---|---|
| Primary Binding Mechanisms | Electrostatic, hydrophobic |
| BSA Adsorption | Not Applicable |
| Wettability | Easily wetted with water |
| Physicochemical Information | |
|---|---|
| Pore Size | 0.45 µM |
| Dimensions | |
|---|---|
| Filter Surface | Plain |
| Filter Dimensions | 8.5 cm x 10 M |
| Materials Information | |
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| Chemistry |
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| Packaging Information | |
|---|---|
| Material Size | 1 |