Cell Signaling Ufd1 Antibody

Name: Cell Signaling Ufd1 Antibody
SKU: CSIG-13789S
Price: 309.06 USD

List Price $309.06 Your Price $309.06

Cell Signaling Ufd1 Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-13789S

MFG.PART: 13789S

UNSPSC: 12352202

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M R Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	40
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:100}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	UFD1 Antibody recognizes endogenous levels of total UFD1 protein.
Species Reactivity	Human, Mouse, Rat, Monkey
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human UFD1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background	The ubiquitin fusion degradation 1 (UFD1) adaptor protein is a component of a protein complex essential for degradation of misfolded proteins by the endoplasmic reticulum-associated protein degradation (ERAD) pathway (1). The UFD1 protein contains a pair of conserved, amino-terminal ubiquitin-binding sites responsible for binding mono- and polyubiquitin molecules (2,3). The carboxy-terminal region of UFD1 contains binding sites for both the adapter protein NPL4 and the AAA ATPase VCP (4). The UFD1-NPL4 heterodimer binds VCP to create a protein complex responsible for export of misfolded proteins from the ER to the cytoplasm for ubiquitin-mediated degradation (5-7). The same protein complex may also be involved in disassembly of the spindle apparatus following mitosis (8).

Specifications