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GenScript Benz-Neburase™, His; 10kU
List Price $39.00 Your Price $39.00
GenScript Benz-Neburase™, His - GSCRPT (Additional S&H or Hazmat Fees May Apply)
NETA PART: GSCRPT-Z03626-10
MFG.PART: Z03626-10
UNSPSC: 12352202
Manufacturer: GenScript USA Inc
Properties
| Expression System | E.coli |
| Species | Serratia Marcescen |
| Tag | N-terminal 6 × His Tag |
| Theoretical Molecular Weight | 27.5 kDa |
| Biological Activity | ≥ 1.1×106U/mg; One unitof Benz-Neburase™, His is defined as the amount of enzyme that causes a ∆A260of 1.0 (equivalent to the complete digestion of 37μg DNA) in 30 min. |
| Formulation | Suppliedas a solution of 20 mM Tris-HCl, 2 mM MgCl2, 20 mM NaCl, 50% Glycerol, pH 8.0. |
| Apparent Molecular Weight | ~27.5 kDa, on SDS-PAGEunder reducing conditions |
| Storage & Stability | This product remains stable for up to 2 weeks at 4 °C or up to 24 months at -20 °C. Avoid repeated freeze-thaw cycles. Do not store below -20 °C! |
Applications
| Thisenzyme can be used to remove all forms of DNA and RNA, including doublestranded, single stranded, linearized, and circular forms. | |
| Theactivity of Benz-Neburase™, His requires 1-2 mM Mg2+. |
Quality Control Specifications
| Purity | ≥ 95% as analyzed by reducing SDS-PAGE |
| Enzyme Activity | ≥250 U/μL |
| Endotoxin Level | ≤ 0.1 EU/kU as determined by gel clotting method. |
Background
| Target Background | The Benz-Neburase is agenetically engineered endonuclease from Serratiamarcescens used as a DNA eraser in the purification processes of biologicalmolecules. The enzyme cleaves all forms of DNA and RNA into smaller nucleotidesof around 5-8 base pairs. Benz-Neburase requires divalent cation, preferably Mg2+for activity, displays a broad pH tolerance, ranging from pH 6 to pH10, optimalat 8-8.5, and has a wide temperature tolerance, ranging from 35℃ to 44℃. The nuclease is a physiologichomodimer and functions more progressively than the monomer. Two disulfidebonds in the nuclease are crucial to its activity and stability. The enzyme isactive in a broad range of conditions and is free of proteolytic activity. Thismakes the enzyme especially useful for applications with challenging DNAcontents such as lysed host cells in viral vector manufacturing processes. |
| Synonyms | alternative to Benzonase® Benzonaseis a registered trademark of Merck KGaA |
| References | 1. Nestle, Marion, and W. K. Roberts. "Anextracellular nuclease from Serratia marcescens: I. Purification and someproperties of the enzyme."Journal of Biological Chemistry244.19(1969): 5213-5218. 2. Benedik, Michael J., and Ulrich Strych."Serratia marcescens and its extracellular nuclease."FEMSmicrobiology letters165.1 (1998): 1-13. 3. Filimonova, Maria N., Kurt L. Krause, andMichael J. Benedik. "Kinetic studies of the Serratia marcescensextracellular nuclease isoforms."Biochemistry and molecularbiology international33.6 (1994): 122-1032. 4. Friedhoff, Peter, et al. "A procedure forrenaturation and purification of the extracellular Serratia marcescens nucleasefrom genetically engineered Escherichia coli."Protein expressionand purification5.1 (1994): 37-43. 5. Franke, Ingo, GregorMeiss, and Alfred Pingoud. "On the Advantage of Being a Dimer, a CaseStudy Using the Dimeric Serratia Nuclease and the Monomeric Nuclease from Anabaenasp. Strain PCC 7120."Journal of Biological Chemistry274.2(1999): 825-832. |
For laboratory research use only. Direct human use,including taking orally and injection and clinical use are forbidden.
| SKU | GSCRPT-Z03626-10 |
|---|---|
| Supplier Part Number | Z03626-10 |
| UM | EA |
| UNSPSC | 12352202 |
| Manufacturer | GenScript USA Inc |
| Temperature | This product remains stable for up to 2 weeks at 4 °C or up to 24 months at -20 °C. Avoid repeated freeze-thaw cycles. Do not store below -20 °C! |
| ProductLine | GSCRPT |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 7 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |