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Millipore The Platelet Derived Growth Factor (Pdgf) Is A Potent Mitogen For A Wide Range Of Cell Types Including Fibroblasts, Smooth Muscle & Connective Tissue. - Mill (Additional S&H or Hazmat Fees May Apply)
Greater than 98% by SDS-PAGE. Endotoxin level is less than 0.1 ng per μg (1EU/μg) of PDGF-BB.
Description
Catalogue Number
GF149
Replaces
GF018
Brand Family
Chemicon®
Trade Name
Chemicon
Description
PDGF-BB, recombinant human
Alternate Names
Platelet Derived Growth Factor-BB
Background Information
Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF which is composed of a dimer of two chains termed the A chain and B chain, can be present as AA or BB homodimers or as an AB heterodimer. Recombinant human PDGF-BB is a 24.3 kDa disulfide-linked homodimer of two B chains (218 total amino acids).
Product Information
Formulation
Presentation
Sterile filtered solution lyophilized from 10 mM HOAc.
Quality Level
MQ100
Applications
Application
The Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle & connective tissue.
Key Applications
Cell Culture
Application Notes
For most in vitro applications, PDGF-BB exerts its biological activity in the concentration range of 0.2 to 10.0 ng/mL. Responding cells are (partial list): fibroblasts, connective tissue.
Biological Information
Purity
Greater than 98% by SDS-PAGE. Endotoxin level is less than 0.1 ng per μg (1EU/μg) of PDGF-BB.
Source
E. coli
Specific Activity
Human PDGF-BB is fully biologically active when compared to standards. The ED50 as determined by its dose-dependent mitogenic activity on BALB/c 3T3 cells is between 6-12 ng/mL.
Gene Symbol
PDGFB
PDGF2
SIS
UniProt Number
P01127
P31240 (mouse)
Q05028 (rat)
Stem Cell Type
Neural Stem Cells
Mesenchymal Stem Cells
Hematopoietic Stem Cells
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
The lyophilized PDGF-BB is stable for a few weeks at room temperature, but best stored at -20°C. After a quick spin, reconstitute in water to a concentration of 0.1-1.0 mg/mL. This solution can be diluted into aqueous buffered solutions and stored at 2-8°C for one week or -20°C for future use. Reconstituted PDGF-BB should be stored in undiluted aliquots at -20°C for up to six months.
