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Millipore The Human Noggin Recombinant Protein Belongs To A Group Of Diffusible Proteins Which Bind To Ligands Of The Tgf-Β Family & Regulate Their Activity By Inhibiting Their Access - Mill (Additional S&H or Hazmat Fees May Apply)
Product is endotoxin free (< 0.1ng/μg of protein (<1EU/μg) and greater than 95% pure by SDS-PAGE gel and HPLC analyses
Description
Catalogue Number
GF173
Description
Noggin Protein, Human Recombinant
Alternate Names
NOG
SYM1
SYNS1
Background Information
Noggin belongs to a group of diffusible proteins which bind to ligands of the TGF-β family and regulate their activity by inhibiting their access to signaling receptors. The interplay between TGF-β ligands and their natural antagonists has major biological significance during development processes, in which cellular response can vary considerably depending upon the local concentration of the signaling molecule. Noggin was originally identified as a BMP-4 antagonist whose action is critical for proper formation of the head and other dorsal structures. Consequently, Noggin has been shown to modulate the activities of other BMPs including BMP-2,-7,-13, and -14. Targeted deletion of Noggin in mice results in prenatal death and recessive phenotype displaying a severely malformed skeletal system. Conversely, transgenic mice over-expressing Noggin in mature osteoblasts display impaired osteoblastic differentiation, reduced bone formation, and severe osteoporosis. Recombinant human Noggin is a 46 kDa disulfide-linked homodimer (120-10C) consisting of two 206 amino acid polypeptide chains. Monomeric glycosylated Noggin migrates at an apparent molecular weight of approximately 28.0-33.0 kDa by SDS PAGE analysis under reducing conditions.
Product Information
Presentation
Product is filtered through a 0.2 micron filter before lyophilization.
Quality Level
MQ100
Applications
Application
The human Noggin recombinant protein belongs to a group of diffusible proteins which bind to ligands of the TGF-β family & regulate their activity by inhibiting their access to signaling receptors.
Biological Information
Purity
Product is endotoxin free (< 0.1ng/μg of protein (<1EU/μg) and greater than 95% pure by SDS-PAGE gel and HPLC analyses
Source
HEK293 cells
Cross Reactivity
Mouse, Chicken
UniProt Number
Q13253
Product Usage Statements
Quality Assurance
Bioactivity assay: Determined by its ability to inhibit 5.0 ng/mL of BMP-4 induced alkaline phosphatase production by ATDC chondrogenic cells. The expected ED50 for this effect is 2.0-3.0 ng/mL of Noggin.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Store at -20°C for up to 6 months from date of receipt.
