Sigma-Aldrich Endothelin 1, Human and Porcine

Research area: Cell Signaling

Endothelin 1 (ET-1) is a peptide hormone[1] located in the endothelial cells[2] and belongs to the endothelin family.[1] It is a derivative of pre-pro-ET-1 which is later converted to mature ET-1 by a group of ET-converting enzymes (ECEs), endopeptidases, chymases, and non-ECE metalloproteinases.[2] The EDN1 gene is mapped to the human chromosome at 6p24.1.[3]

Endothelin 1, Human and Porcine - CAS 117399-94-7 – Calbiochem has been used:

• to study its effects on the production of erythropoietin under normoxia and carbon monoxide-induced hypoxia in rat models[4]
• as a protein kinase C (PKC) agonist to study its effects on the phosphorylation of myofilament proteins in rat myocytes[5]
• to induce a hypertrophic-like state to study its effects on contraction pressure in normal and mutated human induced pluripotent stem cells (hiPSC)-derived cardiomyocytes[6]

Endothelin 1 (ET-1) acts as a mediator of paracrine and autocrine functions. The production of ET-1 is upregulated under pathophysiological conditions and is produced in several types of cells such as cardiac myocytes, vascular smooth muscle cells, and inflammatory cells like leukocytes and macrophages. ET-1 is a potent vasoconstrictor peptide that is stimulated by risk factors in several types of cells for cardiovascular diseases.[2] It is involved in many biological effects such as pro-inflammatory actions, platelet activation, stimulation of free radicals, and mitogenic and proliferative effects.[2] ET-1 plays a role in pulmonary physiology, neurological function, autoimmune disorders, transportation of electrolytes and fluids, and cancer. It is an essential component for the development of embryonic neural crest-derived tissues.[1] ET-1 is involved in osteogenesis by promoting osteoblast proliferation by activating Wnt signaling pathway.[7]

Yes

Toxicity: Standard Handling (A)

H-Cys¹-Ser-Cys³-Ser-Ser-Leu-Met-Asp-Lys-Glu-Cys¹¹-Val-Tyr-Phe-Cys¹⁵-His-Leu-Asp-Ile-Ile-Trp-OH (disulfide bonds: 1 → 15; 3 → 11)

Supplied as an acetate salt.

Dissolve peptide initially at 8 mg/mL in 80% cold acetic acid, then dilute with distilled water to achieve 5% acetic acid and 0.5 mg/mL peptide.

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 2 weeks at -20°C.

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Gresser, O., et Al 1996. Biochem. Biophys. Res. Commun.224, 169.
Murata, S., et Al. 1995. J. Pharmacol. Exp. Ther. 274, 1524.
Hexum, T.D., et Al. 1990. Biochem. Biophys. Res. Commun. 167, 294.
Itoh, Y., et Al. 1988. FEBS Lett. 231, 440.
Yanagisawa, M., et Al. 1988. Nature 332, 411.

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