Sigma-Aldrich Amyloglucosidase From Aspe

Biological Source

Aspergillus niger

Quality Level

100

Form

suspension

Specific Activity

~14 units/mg protein (At 25 °C with glycogen as the substrate; standardized with BSA.)

Mol Wt

M_r97 kDa

Packaging

pkg of 10 mL (10102857001 [100 mg])

Manufacturer/Tradename

Roche

Parameter

55 °C optimum reaction temp.

Optimum Ph

4.6-4.8

Storage Temp.

2-8°C

Related Categories

Roche® Life Science Products

General Description

Amyloglucosidase is synthesized by severalAspergillusgenus species.It is a disaccharidase–type α-glucosidase. This enzyme is an exo-enzyme and one of the major industrial enzymes.The stability of amyloglucosidase can be increased by immobilization.

Specificity

Cleaves terminal glucoses that are α1,4- or α1,6-linked to an oligo- or polysaccharide of multiple glucose units. The product is D-glucose.
Heat inactivation:Heat inactivation is recommended at 80 °C for 45 minutes, followed by rapidly cooling down.

Application

Amyloglucosidase fromAspergillus nigercan be used for the hydrolyzation of terminal α1,4- and α1,6-glucosidic bonds (glucose-glucose bonds) in polysaccharides (e.g., starch, dextrins, glycogen), removing glucose units sequentially from the non-reducing end of the molecule. The enzyme will also cleave maltose and maltosides (maltotriose, maltotetraose,etc.).

Biochem/Physiol Actions

Amyloglucosidase fromAspergillus nigeris capable of hydrolyzing the α-D-(1-4), the α-D-(1-6), and the α-D-(1-3) glucosidic bonds of oligosaccharides.[5] Amyloglucosidase is an extracellular enzyme that converts starch to dextrins and glucose. The enzyme is used in the starch-processing industry for the commercial production of D-glucose from corn syrups.[6][6]

Unit Definition

Unit Conversion: One unit (+25 °C; glycogen as substrate) corresponds to 8.6 U (+60 °C; starch as substrate).

Physical Form

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class

12 - Non Combustible Liquids

Wgk_Germany

WGK 1

Flash_Point_F

does not flash

Flash_Point_C

does not flash