Sigma-Aldrich NADP, 500mg, 24292-60-2, 787.37g/mol, Powder, 2 to 8deg C

Quality Level

100

Assay

≥97%

Form

powder

Mol Wt

M_r743.4
M_r787.4

Packaging

pkg of 1 g (10128058001)
pkg of 100 mg (10128031001)
pkg of 5 g (10240354001)
pkg of 500 mg (10128040001)

Manufacturer/Tradename

Roche

Ph

4.0 ± 1.0(10mg/mL)

Λmax

260 nm

Shipped In

wet ice

Storage Temp.

2-8°C

Related Categories

Roche® Life Science Products

General Description

Nicotinamide adenine dinucleotide phosphate (NADP) and NADPH form a redox pair.The NADPH/NADP ratio regulates the intracellular redox potential, thereby influencing metabolic reactionsin vivo.

Application

The β-NADH phosphate disodium salt has been used for the quantification of glucose in glycogen.It has also been used for the hexokinase assay.

Preparation Note

Storage conditions (working solution):2 to 8 °C
10 mg/ml in aqueous solution, contains at least 99% NADP after 4 weeks at 2 to 8 °C

Analysis Note

Absorbance coefficients:18.0 x 10³[l x mol^-1x cm^{- 1}] at 260 nm, 5.9 x 10³[l x mol^-1x cm^{- 1}] at 333 nm (CN complex)

Absorbance at 340 nm:Imidazol buffer, 100 mM pH 6.7, NADP 2 mM (= 14.87 mg/10 ml) : A₃₄₀approximately 0.100.

Absorbance at 360 nm:Aqueous solution of NADP, 10 mg/ml: A₃₆₀approximately 0.200-0.250.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class

13 - Non Combustible Solids

Wgk_Germany

WGK 1

Flash_Point_F

Not applicable

Flash_Point_C

Not applicable