Sigma-Aldrich Nadph Approx. 98% 500 mg

Quality Level

100

Description

Tetrasodium salt

Assay

97% (dry weight)

Form

powder

Packaging

pkg of 1 g (10621706001)
pkg of 100 mg (10107824001)
pkg of 500 mg (10621692001)

Manufacturer/Tradename

Roche

Λmax

260 and 340 nm

Shipped In

wet ice

Storage Temp.

2-8°C

Smiles String

[Na+].[Na+].[Na+].[Na+].NC(=O)C1=CN(C=CC1)[C@H]2O[C@@H](COP([O-])(=O)OP([O-])(=O)OC[C@H]3O[C@H]([C@H](OP([O-])([O-])=O)[C@@H]3O)n4cnc5c(N)ncnc45)[C@H](O)[C@@H]2O

Related Categories

Roche® Life Science Products

General Description

• Absorbance coefficients: 15.0 x 103 L x mol-1 x cm-1 at 260 nm, 6.3 x 103 L x mol-1 x cm-1 at 340 nm
• Absorbance maxima: 260 nm and 340 nm
• Molecular weight: NADPH-Na4: Mr = 833.4, NADPH: Mr = 745.4
• pH value in water: 8.0 ± 1.0 (10 mg/mL)

Amorphous powder bottled under nitrogen.

Application

NADPH-RO has been used for the determination of thioredoxin reductase activity.

Biochem/Physiol Actions

NADPH is an electron donor and a cofactor for many redox enzymes including nitric oxide synthetase.

Quality

• Purity: =97% ß-NADPH-Na4 based on dry weight (enzymatically and absorbance at 340 nm), =80% (absorbance at 260 nm)
• Organic solvents: =3% (GC)

Preparation Note

Storage conditions (working solution):2 to 8 °C
10 mg/mL in Na-bicarbonate buffer, pH 9, contains at least 95% NADPH after 4 weeks at 2 to 8 °C.

Analysis Note

Absorbance coefficients:15.0 x 10³L x mol^-1x cm^-1at 260 nm, 6.3 x 10³L x mol^-1x cm^-1at 340 nm

Other Notes

For life science research only. Not for use in diagnostic procedures.Packaged based on solid weight.

Storage Class

11 - Combustible Solids

Wgk_Germany

WGK 1

Flash_Point_F

Not applicable

Flash_Point_C

Not applicable