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Sigma-Aldrich Collagenase H 100mg
List Price
$143.42
Your Price
$143.42
Sigma-Aldrich Collagenase H 100mg - SIAL (Additional S&H Or Hazmat Fees May Apply)
NETA PART:
SIAL-11074032001
MFG.PART:
11074032001
UNSPSC:
12352202
Manufacturer:
Sigma-Aldrich
Biological Source
Clostridium histolyticum
Quality Level
100
Sterility
non-sterile
Form
lyophilized
Collagenase Activity
0.15 U/mg lyophilizate
Packaging
pkg of 100 mg (11074032001)
pkg of 2.5 g (11087789001)
pkg of 500 mg (11074059001)
Manufacturer/Tradename
Roche
Concentration
~1 mg/mL (Isolation of rat hepatocytes)
~2 mg/mL (Isolation of adipocytes)
Technique(S)
tissue processing: suitable
Color
brown
Related Categories
Collagenase
Roche® Life Science Products
Tissue Dissociation
General Description
Collagenase H is an enzyme mixture prepared fromClostridium histolyticumcultures by filtration, ammonium sulfate precipitation, dialysis, and lyophilization.Specificity
Collagenase degrades native collagen. Clostripain, trypsin-like enzymes, and neutral proteases also degrade other proteins.Application
Collagenase fromC. histolyticumis used for the dissociation of tissues for the establishment of primary cell cultures. Collagenase H is well suited for the isolation of hepatocytes from rat liver by the collagenase perfusion method. This enzyme preparation is also suitable for the preparation of other types of cells such as endothelial cells from large vessels, and for the isolation of adipocytes from epididymal fat pads of rats.Physical Form
Lyophilizate, nonsterilePreparation Note
Enzyme activity:Collagenase activity: >0.15U/mg (according to Wünsch) (+25°C, 4-phenyl-azobenzyl-oxycarbonyl-Pro-Leu-Gly-Pro-D-Arg as the substrate).
Contaminating enzyme activities: trypsin, clostripain, and total proteolytic activity
Collagenase H has a balanced ratio of enzyme activities, and is function tested for the isolation of rat hepatocytes (perfusion method).
Inhibitors:
Collagenase inhibitors: EDTA, EGTA, Cys, His, DTT, 2-mercaptoethanol
Collagenase is not inhibited by serum.
Clostripain inhibitors: TLCK
Trypsin inhibitors: aprotinin, trypsin inhibitor (egg white, soybean)
Activator:Ca2+
Working concentration:0.5 to 2.5mg/ml,
Approximately 1 mg/ml for the isolation of rat hepatocytes and 2mg/ml for the isolation of adipocytes.
Storage conditions (working solution):-15 to -25°C
The reconstituted solution should be stored at -15 to -25°C for short term storage, -60°C or as described below for long-term storage.
Reconstitution
Reconstitution in any balanced salt solution (e.g., HBSS)Storage And Stability
Keep container tightly closed in a dry and well-ventilated place.Other Notes
For life science research only. Not for use in diagnostic procedures.Pictograms
GHS07,GHS08Signalword
Danger
Hcodes
H315 - H319 - H334 - H335Pcodes
P261 - P264 - P280 - P284 - P304 + P340 + P312 - P342 + P311Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target_Organs
Respiratory system
Storage Class
11 - Combustible Solids
Wgk_Germany
WGK 1
Flash_Point_F
does not flash
Flash_Point_C
does not flash
| SKU | SIAL-11074032001 |
|---|---|
| Featured | No |
| Supplier Part Number | 11074032001 |
| UM | EA |
| UNSPSC | 12352202 |
| Manufacturer | Sigma-Aldrich |
| Temperature | 2-8C |
| Refrigerated-Frozen | Y |
| ProductLine | SIAL |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 10.000000 |
| Lead Time | 9 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| ACT Ecolabel | No |
| Controlled | N |