Sigma-Aldrich Collagenase H 500mg

Biological Source

Clostridium histolyticum

Quality Level

100

Sterility

non-sterile

Form

lyophilized

Collagenase Activity

0.15 U/mg lyophilizate

Packaging

pkg of 100 mg (11074032001)
pkg of 2.5 g (11087789001)
pkg of 500 mg (11074059001)

Manufacturer/Tradename

Roche

Concentration

~1 mg/mL (Isolation of rat hepatocytes)
~2 mg/mL (Isolation of adipocytes)

Technique(S)

tissue processing: suitable

Color

brown

Related Categories

Collagenase

Roche® Life Science Products

Tissue Dissociation

General Description

Collagenase H is an enzyme mixture prepared fromClostridium histolyticumcultures by filtration, ammonium sulfate precipitation, dialysis, and lyophilization.

Specificity

Collagenase degrades native collagen. Clostripain, trypsin-like enzymes, and neutral proteases also degrade other proteins.

Application

Collagenase fromC. histolyticumis used for the dissociation of tissues for the establishment of primary cell cultures. Collagenase H is well suited for the isolation of hepatocytes from rat liver by the collagenase perfusion method. This enzyme preparation is also suitable for the preparation of other types of cells such as endothelial cells from large vessels, and for the isolation of adipocytes from epididymal fat pads of rats.

Physical Form

Lyophilizate, nonsterile

Preparation Note

Enzyme activity:
Collagenase activity: >0.15U/mg (according to Wünsch) (+25°C, 4-phenyl-azobenzyl-oxycarbonyl-Pro-Leu-Gly-Pro-D-Arg as the substrate).
Contaminating enzyme activities: trypsin, clostripain, and total proteolytic activity
Collagenase H has a balanced ratio of enzyme activities, and is function tested for the isolation of rat hepatocytes (perfusion method).
Inhibitors:
Collagenase inhibitors: EDTA, EGTA, Cys, His, DTT, 2-mercaptoethanol
Collagenase is not inhibited by serum.
Clostripain inhibitors: TLCK
Trypsin inhibitors: aprotinin, trypsin inhibitor (egg white, soybean)
Activator:Ca²⁺
Working concentration:0.5 to 2.5mg/mL,
Approximately 1 mg/mL for the isolation of rat hepatocytes and 2mg/mL for the isolation of adipocytes.
Storage conditions (working solution):-15 to -25°C
The reconstituted solution should be stored at -15 to -25°C for short term storage, -60°C or as described below for long-term storage.

Reconstitution

Reconstitution in any balanced salt solution (e.g., HBSS)

Storage And Stability

Keep container tightly closed in a dry and well-ventilated place.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Pictograms

GHS07,GHS08

Signalword

Danger

Hcodes

H315 - H319 - H334 - H335

Pcodes

P261 - P264 - P280 - P284 - P304 + P340 + P312 - P342 + P311

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target_Organs

Respiratory system

Storage Class

11 - Combustible Solids

Wgk_Germany

WGK 1

Flash_Point_F

does not flash

Flash_Point_C

does not flash