Sigma-Aldrich N-Glycosidase F 100 U

Recombinant

expressed inE. coli

Quality Level

100

Conjugate

(N-linked)

Assay

≥90% (SDS-PAGE)

Form

solution

Specific Activity

~25000 units/mg protein

Packaging

pkg of 0.1 mL (11365169001 [100 U])
pkg of 0.25 mL (11365177001 [250 U])

Manufacturer/Tradename

Roche

Optimum pH

7.0-8.0

Storage Temp.

−20°C

Related Categories

Glycobiology Profiling Tools

Roche® Life Science Products

General Description

Peptide-N-glycosidase F, Peptide-N⁴-(acetyl-β-glucosaminyl)-asparagine amidase

Application

Use N-glycosidase F to cleave all types of asparagine-bound N-glycans, provided that the amino group as well as the carboxyl group are present in a peptide linkage, and that the oligosaccharide has the minimum length of the chitobiose core unit.The reaction products are ammonia, aspartic acid (in the peptide chain), and the complete oligosaccharide.
Note: N-Glycosidase F, recombinant is also available as a lyophilizate without glycerol.

Unit Definition

One unit is the enzyme activity which hydrolyzes 1 nmol dabsyl fibrin glycopeptide or 0.2 nmol dansyl fetuin glycopeptide within 1 minute at 37 °C at pH 7.8.

Physical Form

Solution in 50 mm sodium phosphate, 12.5 mm EDTA, 50% glycerol (v/v), pH 7.2

Other Notes

For life science research only. Not for use in diagnostic procedures.The sale of the Product does not exhaust or grant any rights in third party patents including patents of companies of the F. Hoffmann - La Roche AG group of companies, in particular, for the use of modified antibodies obtained by using the product.