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Sigma-Aldrich Albumin, Bovine Serum, Fraction V, Fatty Acid-Free
List Price
$739.02
Your Price
$739.02
Sigma-Aldrich Albumin Bovine Serum Fract 1pc X 100gm - SIAL (Additional S&H Or Hazmat Fees May Apply)
NETA PART:
SIAL-126575-100GM
MFG.PART:
126575-100GM
UNSPSC:
12352202
Manufacturer:
Sigma-Aldrich
assay
≥95% (cellulose acetate electrophoresis)
form
powder
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
impurities
≤0.02% fatty acids
≤3% ash
≤5% moisture
color
white to light yellow
light yellow to light brown
shipped in
ambient
Storage temp.
2-8°C
Related Categories
Albumins and Transport Proteins
Bovine Serum Albumin
General description
Albumin, Bovine Serum, (BSA) Fraction V, Fatty Acid-Free is useful for immunodiagnostic assays. It is designed for use in serological testing, radioimmunoassay, and hormone response studies. It is also applicable in binding and transport studies. BSA is prepared by cold ethanol extraction (Cohn method) with a pH: 6.8-7.4.
Bovine serum albumin (BSA) is a non-glycosylated globular, α-helical protein belonging to the serum albumin family.[1][2] It consists of three domains with two sub-domains each and has 17-disulfide bonds.[1][2]
Application
Albumin, Bovine Serum, Fraction V, Fatty Acid-Free has been used:
- as a component of: blocking buffer immunoelectron microscopy (IEM) for various cells[3]
- mitochondrial isolation buffer (MIB) and mitochondria assay solution (MAS) to trap free fatty acids released during adipose tissue homogenization[4]
- blocking buffer for wholemount staining of the esophageal epithelium (EE) in immunofluorescence[5]
Biochem/physiol Actions
Bovine Serum Albumin (BSA), the most vital component of cell culture media, aids in embryonic stem cells (hESC) differentiation.[6] It aids the transportation of drugs, hormones, and fatty acids.[1] BSA is also used as a blocking agent in enzyme-linked immunosorbent assay (ELISA).[7]
Warning
Toxicity: Standard Handling (A)
Preparation Note
May take up to 24 h to fully dissolve.
Reconstitution
Following reconstitution, store in the refrigerator (4°C). 0.1% NaN₃ may be added as a preservative. Stock solutions are stable for up to 3 months at 4°C.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
wgk_germany
WGK 3
| SKU | SIAL-126575-100GM |
|---|---|
| Featured | No |
| Supplier Part Number | 126575-100GM |
| UM | EA |
| UNSPSC | 12352202 |
| Manufacturer | Sigma-Aldrich |
| Temperature | CL |
| ProductLine | SIAL |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 10.000000 |
| Lead Time | 9 |
| Hazardous | N |
| CAS Number | 9048-46-8 |
| Energy Star | No |
| Green | No |
| ACT Ecolabel | No |
| Controlled | N |