Sigma-Aldrich Sodium Orthovanadate Activ

Form

liquid

Quality Level

100

Manufacturer/Tradename

Calbiochem^®

Storage Condition

OK to freeze
avoid repeated freeze/thaw cycles
protect from light

Color

colorless

Storage Temp.

−70°C

General Description

A depolymerized vanadate prepared from Sodium Orthovanadate (>Cat. No. 567540) that exhibits significant enhanced ability to inhibit endogenous phosphatases in a competitive manner. Preserves the phosphorylation state in cells and cell lysates. Completely reversible with EDTA and dilution. Recommended concentration in lysis buffer ~ 1 mM. Available as 5 ml or as a set of 3 x 1 ml.

Biochem/Physiol Actions

Primary Target
PhosphatasesReversible: yes

Packaging

Packaged under inert gas

Warning

Toxicity: Toxic (F)

Physical Form

Supplied as 10 mg/ml in aqueous NaOH, pH 10.0.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C). Aliquots are stable for up to 1 year at -70°C.

Other Notes

Gordon, J.A. 1984.Methods. Enzymol. 201,477.
Brown, D.J., and Gordon, J.A. 1984.J. Biol. Chem. 259,9580.CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany