Tonbo Classic++ Hot Start Taq Dna Polymerase

Tonbo Classic++ Hot Start Taq DNA Polymerase Cat.No. 31-5010-0250U Size 250 units; Classic++ Hot Start Taq DNA Polymerase is Tonbos next generation thermostable Taq DNA polymerase of recombinant origin that possesses 53 polymerase activity, but not 3__5_ proofreading, exonuclease activity. In addition, two proprietary Classic++ Hot Start mAbs (monoclonal antibodies) have been developed which are used to specifically block polymerase activity below 70’°C, allowing for convenient room temperature reaction set up. DNA polymerase activity is restored during the initial denaturation step when amplification reactions are heated at 94-95’°C for two minutes. The blocking of polymerase activity prior to denaturation of template improves yields by minimizing or eliminating primer dimer formation and non-specific amplification. For added convenience and value, the advanced Reaction Buffer (5X) includes dNTPs and MgCl2. Classic++ Hot Start Taq polymerase is ideal for a wide variety of fast or standard PCR protocols when additional speed, sensitivity, specificity and yields are desired.Classic++ Hot Start Taq polymerase has a non-template-dependent terminal transferase activity that adds a 3 A overhang to the fragment, useful for downstream TA cloning. This hot start polymerase may be used in a wide variety of applications including genotyping, multiplexing, general colony screening and library construction with little to no protocol modification. Use Classic++ Hot Start Taq DNA Polymerase for the amplification of DNA from GC and AT rich regions from complex genomic, viral, and plasmid templates, as well as in RT-PCR. - TB (Additional S&H or Hazmat Fees May Apply)