Tonbo Classic++ Hot Start Taq Dna Polymerase Master Mix

Tonbo Classic++ Hot Start Taq DNA Polymerase Master Mix Cat.No. 31-5011-0200R Size 200 rxns; Classic++ Hot Start Taq DNA Polymerase Master Mix is a premixed, ready-to-use cocktail optimized for efficient amplification from a wide variety of DNA templates. Tonbos Classic++ Hot Start Taq DNA Polymerase is a next generation thermostable DNA polymerase of recombinant origin that possesses 53 polymerase activity, but not 3__5_ proofreading, exonuclease activity. The enzyme has been further modified by the temperature dependent inclusion of two proprietary Classic++ Hot Start mAbs (monoclonal antibodies) that act in concert to specifically block polymerase activity below 70’°C, allowing for convenient room temperature reaction set up. DNA polymerase activity is restored during the initial denaturation step when amplification reactions are heated at 94-95’°C for two minutes. The blocking of polymerase activity prior to denaturation of template improves yields by minimizing or eliminating primer dimer formation and non-specific amplification. Convenient and reliable, Classic++ Hot Start Taq DNA Polymerase is ideal for standard PCR protocols and has been engineered to provide enhanced speed, yield and specificity over that of wild-type and standard forms of Taq DNA polymerase. In the presence of Tonbos optimized Master Mix and your primer(s), this hot start enzyme will synthesize double-stranded DNA from a wide variety of single stranded templates.Tonbo provides a robust, convenient, high performance Master Mix optimized to enhance standard PCR speed, yield and specificity. Classic++ Hot Start Taq polymerase has a non-template- dependent terminal transferase activity that adds a 3 A overhang to the fragment, useful for downstream TA cloning. This hot start polymerase may be used in a wide variety of applications including genotyping, multiplexing, general colony screening and library construction with little to no protocol modification. Use Classic++ Hot Start Taq DNA Polymerase for the amplification of DNA from GC and AT rich regions from complex genomic, viral, and plasmid templates, as well as in RT-PCR. - TB (Additional S&H or Hazmat Fees May Apply)