Tonbo Veritypfu Dna Polymerase

Tonbo VerityPfu DNA Polymerase Cat.No. 31-5020-0200U Size 200 units; VeriftyPfu DNA Polymerase is a proprietary next generation, high fidelity, proofreading enzyme. It replicates DNA at 75’°C, catalyzing template dependent polymerization of free nucleotides into duplex DNA in the 53 direction in the presence of magnesium ions. The VerityPfu DNA polymerase was developed using site directed mutagenisis that allowed for the positive selection of a very high fidelity Pfu variant optimized for use at room temperature. Accordingly, the VerityPfu enzyme possesses exceptional 3-5 exonuclease, or proofreading, activity that results in 50X higher fidelity than standard Taq DNA polymerase. It does not require hot start conditions, allowing easy room temperature PCR reaction set up. Consequently, VerityPfu polymerase, when used together with its proprietary buffer system, is highly recommended for use in reactions that require high fidelity synthesis. For added convenience and value, the advanced Reaction Buffer (5X) includes dNTPs and MgCl2.Due to genetic mutations conferring exceptional proofreading capabilities, VerityPfu polymerase is especially effective when very high levels of fidelity are required. This polymerase reliably generates amplicons up to 10 kb under both fast and standard PCR cycling conditions. The resultant amplicons are blunt-end finished and can be used in a variety of downstream applications. Use VerityPfu DNA Polymerase to accurately and reliably amplify DNA from complex genomic, viral, and plasmid templates, and for site directed mutagenesis, long range PCR and next generation sequencing applications, with little to no protocol modification. - TB (Additional S&H or Hazmat Fees May Apply)