Tonbo Veritymax Dna Polymerase Master Mix

Tonbo VerityMAX DNA Polymerase Master Mix Cat.No. 31-5031-0080R Size 80 rxns; Tonbos VerityMAX DNA Polymerase Master Mix contains a proprietary blend of VeriftyPfu and Classic++ Hot Start Taq DNA polymerases, engineered and optimized specifically for generating amplicons of up to 35 kb in length with 2.5x higher fidelity than standard Taq polymerase. The VerityPfu polymerase was developed using site directed mutagenesis that allowed for the positive selection of a very high fidelity Pfu variant optimized for use at room temperature. Accordingly, the VerityPfu enzyme possesses exceptional 3-5 exonuclease, or proofreading, activity. Classic++ Hot Start Taq DNA Polymerase is Tonbos next generation recombinant Taq polymerase that possesses 53 exonuclease activity. Proprietary Classic++ Hot Start mAbs (monoclonal antibodies) have been developed that specifically block polymerase activity below 70’°C, allowing for convenient room temperature reaction set up. Hot start technology improves yields by minimizing or eliminating primer dimer formation and non-specific amplification. Tonbos VerityMAX DNA Polymerase Master Mix is ideal for a wide variety of fast or standard PCR protocols when additional speed, sensitivity, specificity and yields are desired. Use the VerityMAX Master Mix to accurately and reliably amplify DNA of up to 35 kb from complex GC/AT rich genomic DNA, plasmid templates, crude PCR and for bacterial colony PCR. The resultant amplicons are both blunt-end finished and A-tailed and can be used in a wide variety of downstream applications, including TA and blunt-end cloning. - TB (Additional S&H or Hazmat Fees May Apply)