Tonbo Classic++ 1-Step Rt-Pcr Kit

Tonbo Classic++ 1-Step RT-PCR Kit Cat.No. 31-5220-0050R Size 50 rxns; The Classic++ 1-Step RT-PCR Kits advanced reagents allow for efficient cDNA synthesis and PCR in a single tube. Included in the Master Mix is Classic++ Hot Start Taq DNA polymerase, a next generation thermostable polymerase of recombinant origin that possess 53 polymerase activity. It also contains two proprietary Classic++ Hot Start mAbs (monoclonal antibodies) which are used to specifically block polymerase activity below 70’°C, allowing for convenient room temperature reaction set up. Following successful reverse transcription of RNA target molecules, DNA polymerase activity is restored during the initial denaturation step when amplification reactions are heated at 94-95’°C for two minutes. The blocking of polymerase activity prior to denaturation of template improves yields by minimizing or eliminating primer dimer formation and non-specific amplification. In addition, a proprietary 20X M-MLV Reverse Transcriptase (RT) molecule blended with an optimized RNase inhibitor is included in a separate tube. The enzyme can synthesize cDNA at a temperature range of 45-60’°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases. Our M-MLV RT is not significantly inhibited by ribosomal and transfer RNA, making it ideal to synthesize cDNA from total RNA. RT-PCR (reverse transcription-polymerase chain reaction) is used to convert and amplify a single-stranded RNA template to yield abundant double-stranded complementary DNA (cDNA) product. One-step RT-PCR is a variation on standard RT-PCR in which all reaction components are mixed in one tube prior to starting the reactions. This approach offers simplicity and convenience and minimizes the possibility for contamination.The Classic++ 1-Step RT-PCR Kit is designed for the sensitive, reproducible, end-point detection and analysis of mRNA or total RNA molecules by RT-PCR. Our convenient formulation allows for high speed, accurate and reproducible cDNA synthesis and subsequent PCR amplification in a single tube using gene-specific primers, starting from a variety of target RNA templates. - TB (Additional S&H or Hazmat Fees May Apply)