Sigma-Aldrich Expand Hifi Plus PCR Sys.

Usage

sufficient for ≤2,500 reactions (03300234001)
sufficient for 2500 reactions
sufficient for ≤500 reactions (03300226001)
sufficient for 500 reactions

Quality Level

100

Feature

High Fidelity PCR
dNTPs included: no
hotstart: no

Packaging

pkg of 2,500 U (03300234001 [10x250 U])
pkg of 500 U (03300226001 [2x250 U])

Manufacturer/Tradename

Roche

Parameter

72 °C optimum reaction temp.

Technique(S)

PCR: suitable

Input

purified DNA

Storage Temp.

−20°C

Related Categories

PCR Reagents & Kits

Roche® Life Science Products

General Description

The Expand High Fidelity^PLUSPCR System is a blend of Taq DNA polymerase and a thermostable proofreading protein that lacks polymerase activity.
The Expand High Fidelity^PLUSPCR System is designed to amplify fragments up to 5 kb from all types of DNA with high yield and fidelity. It amplifies DNA with sixfold greater fidelity than Taq DNA polymerase alone.
Expand High Fidelity^PLUSPCR System also incorporates dUTP. Cross-contamination can therefore be prevented by a pretreatment of dUTP containing DNA with heat-labile Uracil-DNA Glycosylase before amplification. It is suited for the simultaneous amplification of a large number of targets.

Application

The Expand^™High Fidelity^PLUSPCR System designed for the robust amplification of fragments up to 5 kb from all types of DNA with outstanding yield, specificity, sensitivity, and accuracy. This kit is the product of choice for the development of a new amplification assay or the optimization of an existing assay.
Use Expand High FidelityPLUS PCR System for:

• PCR
• RT-PCR
• Primer extension
• Carryover prevention

Features And Benefits

• Safeguard against cross-contamination.

This is the choice for large numbers of amplifications.

• Obtain sixfold higher fidelity and higher yields,

compared to Taq DNA Polymerase.

• Efficiently label DNA.

Use this kit to label DNA with radioactive or nonradioactive nucleotides.

Packaging

1 kit containing 4 components

Quality

Each lot is PCR function-tested using human genomic DNA and EPO primers specific for a 1.8kb PCR product, and tPA primers specific for a 4.8kb PCR product.

Unit Definition

Volume Activity: 5 U/μL

Other Notes

For life science research only. Not for use in diagnostic procedures.Transition-metal-catalyzed cross-coupling is an efficient tool for constructing C-C, C-N and C-O bonds in organic synthesis. Intensive interest has been focused on the development of the most effective ligands that improve catalyst performance. Ligands play a key role in stabilizing and activating the central metal atom and fine-tuning the selectivity of transformation. Phosphines have proven to be the most significant class of ligands for cross couplings, such as Negishi, Suzuki, Stille, Heck, Sonogashira, and Buchwald-Hartwig cross-coupling.Expand is a trademark of RocheH412P273 - P501