Revvity HTRF Human and Mouse Phospho-Cofilin Ser3 Detection Kit, 500 Assay Points

The kit is designed for the rapid detection of Phospho Cofilin Ser3 in cell supernatant and whole cells. Actin-binding proteins are abundant cellular proteins that regulate cell function by mediating actin polymerization and remodeling. Cofilin, known as a regulator of actin filament dynamics, is a small ~21kDA protein that is ubiquitously expressed in all vertebrates and freely diffuses in eukaryotic cells. Phosphorylation at Ser-3 by kinases attenuates cofilinâs actin- binding activity. In contrast, dephosphorylation at Ser-3 enhances cofilin-induced actin depolymerization.