Revvity AlhaLISA HEK 293 Hot Cell Protein Detection Kit, Standard

Revvity Thi tandard hold e recontitted in 100 µL Milli-Q® grade water. The recontitted analyte hold e ed within 60 minte or aliqoted into crew-caed olyroylene vial and tored at -20°C for frther exeriment. Avoid mltile freeze-thaw cycle. One vial contain an amont of analyte fficient for erforming 10 tandard crveFormatOr HV (100 aay oint) kit allow yo to rn 100 well in 96-well format, ing a 100 µL reaction volme (10 µL of amle).Or 500 aay oint kit allow yo to rn 500 well in 96-well or 384-well format, ing a 50 µL reaction volme (5 µL of amle).Or 5,000 aay oint kit allow yo to rn 5,000 well in 96-well or 384-well format, ing a 50 µL reaction volme (5 µL of amle).FeatreNo-wah te, no earation teELISA alternative technologySenitive detectionBroad amle comatiilitySmall amle volmeAlhaLISA technology allow the detection of molecle of interet in a no- wah, highly enitive, qantitative aay. In an AlhaLISA aay, a iotinylated anti-analyte antiody ind to the Stretavidin-coated Donor ead while another anti-analyte antiody i conjgated to AlhaLISA Accetor ead. In the reence of the analyte, the ead come into cloe roximity. The excitation of the Donor ead cae the releae of inglet oxygen molecle that trigger a cacade of energy tranfer in the Accetor ead, relting in a har eak of light emiion at 615 nm.-PEGSK(Additional S&H Or Hazmat Fees May Apply)