Revvity AlphaLISA SureFire Ultra Human Phospho-CDC6 (Ser54) Detection Kit, 100 Assay Points

Cell division cycle 6 (CDC6) is an essential licensing factor in eukaryotic cells for DNA replication during G1 phase and S phase. CDC6 is known for its assembly of pre-replicative complexes at origins of replication during G1 phase, making it a key player in DNA replication. Numerous studies have shown that abnormal CDC6 expression is involved in oncogenic activities.

The AlphaLISA SureFire Human Phospho-CDC6 (Ser54) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-CDC6 (Ser54) in cellular lysates, using Alpha Technology.

Formats

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for

• Cellular kinase assays
• Receptor activation studies
• Screening