Revvity Alphalisa Surefire Ultra Human And Mouse Total Irf5 Detection Kit, Control Lysate

Revvity Thi control lyate i intended for e with the AlhaLISA SreFire Ultra Hman and Moe Total IRF5 Detection Kit or for e a a control for thoe rnning orthogonal or econdary aay along with the SreFire aay. To reare the lyate, THP-1 cell were eeded at 300KmL, and incated for 48 hor in T175 flak in 10% FBS containing medim. Cell were harveted and reended at 8 x 106 cellmL and treated with 100 nM Calyclin A for 3 hor. Cell were then wahed in HBSS + 0.1% BSA and lyed in Lyi Bffer at a final cell denity of 8 x 106 cellmL.-PEGSK(Additional S&H Or Hazmat Fees May Apply)