Revvity HTRF Human/Mouse Total CREBBP Detection Kit, 500 Assay Points

The HTRF Total CREBBP cell-based assay conveniently and accurately detects the levels of Total CREBBP within cells. This kit is compatible with the buffers from the Total p300 kit, SO the same lysate can be used for analyses of both family members.

CREBBP (CREB Binding Protein, also known as CBP or KAT3A) and its paralog p300 (also called EP300 or KAT3B) are key transcriptional co-activators. These Lysine Acetyl Transferases (KATs) form the two-member KAT3 family. They control gene transcription by facilitating the assembly of transcriptional machinery and by acetylating specific lysine residues on core histones and on transcription coregulators. CREBBP and p300 act as network "hubs", with more than 400 proteins to regulate the expression of genes controlling several basic cellular processes, such as proliferation and homeostasis.

Mutations or chromosomal translocations involving CREBBP/p300 lead to gene dysregulation and can result in a variety of human diseases such as cancer, neurodegeneration, and inflammatory diseases. CREBBP/p300 PROTAC degraders have recently been developed for the treatment of human cancers.