Revvity HTRF Human Phospho-FLT3 Tyr589/591 Detection Kit, 10,000 Assay Points

The HTRF Phospho Tyr 589/591 Human FLT3 (Fms-like tyrosine kinase 3) detection assay, also named FLK2, monitors Wild-Type FLT3 (FLT3 WT) and/or Internal Tandem Duplication (ITD) FLT3 mutant phosphorylation at Tyr 589/591 in various cells. FLT3 is a class III receptor tyrosine kinase expressed in immune cells and activated by its ligand, FLT3L. After FLT3L binding, the FLT3 receptor dimerizes and activates a variety of pathways, including the phosphatidylinositol 3-kinase (PI3K) and the MAPK pathways resulting in cell division, proliferation, and survival of immune cells. A dysregulation of the FLT3 pathways could lead to anarchic proliferation, immature immune cells, and oncogenic phenotypes. FLT3 is the most commonly mutated gene in Acute Myeloid Leukemia (AML). Mutations in the FLT3 gene lead to constitutive activation of the receptor kinase activity, or disrupt the autoinhibitory activity of the receptor. The ITD FLT3 mutant is well known for its implication in AML. FLT3 mutants have therefore become important drug targets for the pharmaceutical industry.