Revvity HTRF Human/Mouse Total p300 Detection Kit, 10,000 Assay Points

The Total p300 cell-based assay conveniently and accurately detects the levels of Total p300 within cells. This kit is compatible with the buffers from the Total CREBBP kit, so the same lysate can be used for analyses of both family members.

p300 (also called EP300 or KAT3B) and its paralog CREBBP (CREB Binding Protein, also known as CBP or KAT3A) are key transcriptional co-activators. These Lysine Acetyl Transferases (KATs) form the two-member KAT3 family. They control gene transcription by facilitating the assembly of transcriptional machinery and by acetylating specific lysine residues on core histones and on transcription coregulators. p300 and CREBBP act as network "hubs", with more than 400 proteins to regulate the expression of genes controlling several basic cellular processes, such as proliferation and homeostasis.

Mutations or chromosomal translocations involving p300/CREBBP lead to gene dysregulation and can result in a variety of human diseases such as cancer, neurodegeneration, and inflammatory diseases. p300/CREBBP PROTAC degraders have recently been developed for the treatment of human cancers.