Revvity Alphalisa Surefire Ultra Human Phospho-Rab29 (Ser72) Detection Kit, Control Lysate

Revvity this control lysate is intended for use with the alphalisa surefire ultra human phospho-rab29 (ser72) detection kit or for use as a control for those running orthogonal or secondary assays along with the surefire assay. to prepare the lysate, thp-1 cells were seeded at 500k/mL in fresh medium for 24 hours. cells were treated with 100 ng/mL calyculin for 2 hours. cells were spun down at 300 rcf for 5 minutes, washed with HBSS and lysed with lysis buffer at 10x106 cells/mL. - PEMSD (Additional S&H Or Hazmat Fees May Apply)